中国园艺学会第七届青年学术讨论会

中国园艺学会第九届第8次常务理事扩大会决定,“中国园艺学会第七届青年学术讨论会”由山东农业大学园艺科学与工程学院和山东省园艺学会承办,将于2006年7月或8月在山东泰安举行。会议交流主题:(1)园艺作物种质资源、遗传育种与生物技术;(2)园艺作物有机、无公害及标准化安全生

Effects of berberine on proliferation and apoptosis of HUVECs

AIM: To investigate the mechanism of berberine’s inhibiting growth and metastasis of tumor by observing the effects of berberine on proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs). METHODS: Cultured HUVECs were incubated with berberine. MTT assay and quantitative immunocytochemistry were used to detect cell proliferation and the expression of proliferation cell nuclear antigen (PCNA). Morphologic changes of apoptosis were observed by fluorescent staining, and Rhodamin123 was used to determinate mitochondrial membrane potential under the laser confocal microscopy. RESULTS: HUVEC proliferation was inhibited by co-incubating with berberine (20 mg/L) for 24 h and berberine (10 mg/L, 20 mg/L) for 48 h （P<0.01, P<0.05，P<0.01）. In the presence of berbering (20 mg/L) for 48 h, the expression of PCNA in HUVECs was decreased significantly （P<0.01）, typical apoptotic appearance was found and mitochondrial membrane potential declined markedly （P<0.01）. CONCLUSIONS: Berberine has a significant inhibitory effect on proliferation of HUVECs and an induced effect on HUVEC apoptosis, which is related with mitochondria. Berberine inhibits the proliferation of vascular endothelial cells to suppress neovascularization, which may be one of the mechanisms underlying inhibitory effect of berberine on growth and metastasis of tumor.