东北大豆品种贮藏蛋白7S和11S组分及其亚基相对含量分析

利用聚丙烯酰胺凝胶电泳分析了黑龙江、吉林、辽宁和内蒙古4省(区)近10 a来通过审定的163个大豆品种的11s和7s球蛋白亚基组成、相对百分含量和11s/7S比值,并对其进行了省份问比较分析和相关性分析.结果表明:品种问大豆蛋白各亚基的相埘含量存在较大差异;163份供试材料的7s、11S球蛋白平均相对含量分别为28.95%和56.30%.变幅分别为18.40%～36.20%和47.90%～71.50%,11S/7S比值的变异幅度在1.34～3.32之间,平均值为1.97;初步筛选出α和A3缺失或稀少的特异大豆品种9份;吉林、黑龙江、辽宁和内蒙古4个地区大豆群体蛋白亚基组成及11S/7S比值存在显著差异.7S和11S组分含量问存在极显著的负相关(r=-0.2862,P<0.01).东北大豆品种贮藏蛋白7S和11S组分及其亚基相对含量存在显著变异,为大豆品质育种和豆制品加工业原料选择提供了丰富的物质基础.

Analysis of 7S and 11S Globulin and Subunit Content of Soybean in Northeast China

The contents of 7S and US globulins and their respective sulxniits,and the 11S/7S ratios were tightly associated with the nutrition and processing properties of soybean protein. The relative percentage content of US and 7S and the 11S/7S value of 163 soybean cultivars from Northeast China were investigated by SDS-PAGE method. Results showed the content of protein subunits varied greatly among varieties, the average content and variation rage of 11S and 7S globulin were 56.30% and 28.95% ,47.90%-71.50% and 18. 40%-36. 20% .respectively; 11S/7S value ranged from 1.34 to 3. 32, with an average of 1. 97 ;nine accessions screened from 163 germplasm showed polymorphism of band type for the subunit relative contents. 11S/7S value was significantly different among the four cultivated regions; remarkably negative correlation existed between the relative contents of 7S and 1 IS globulins(r =-0.2862, P <0.01). Results suggest the subunit composition and their relative content of the storage protein 7S and US from Northeast China had remarkable variation, which would provid rich material for the soybean quality breeding.