猪伪狂犬病病毒囊膜糖蛋白E基因的序列结构分析

对猪伪狂犬病毒鲁A株(PRVLA株)gE基因进行了克隆和序列测定。应用DNAStar程序分析了PRV国内外各分离株以及PRV与HSV-1、BHV-1、EHV-1、CHV-1、MDV-1、SHV-SA8、SVV、ILTV的gE基因。PRV各分离株的gE基因有很高的同源性。不同疱疹病毒的gE核苷酸和氨基酸的同源性很低,但具有相似的结构。对上述α疱疹病毒的gE氨基酸序列进化树分析可以将其分为四个特定的组:单纯疱疹病毒组,水痘疱疹病毒组,类马立克氏病病毒组,传染性喉气管炎病毒组,推测α疱疹病毒的gE基因在进化上可能起源于ILTV的gE基因的或其前体基因。而且gE基因的进化趋势与其宿主的进化情况一致。

Phylogenetic Tree and Evolution Analysis of gE Gene of Pseudorabies Virus

The gE gene of PRV La strain is amplified and sequenced. The ORF of PRV LA gE gene is 1740bp-coding glycoprotein E. The homology of deduced amino acids of PRV La strain with other PRV strains is high. The variance of PRV gE sequence shows regular regionally. PRV gE genes shared limited homology to other alphaherpesviruses equivalents: HSV-1?BHV-1?EHV-1?CHV-1?SHV-SA8?SVV?MDV-1 and ILTV. The homology analysis of deduced amino acids of above alphaherpesviruses equivalents showed that gE could be separated into 4 groups: simplex?varicella?Marek's disease-like viruses and infectious laryngotracheitis virus group,the phylogenetic tree analysis contribute further evidence of the evolution of gE of alphaherpesviruses from a common ILTV gE progenitor,and evolutional trend of gE conforms with the evolution of their hosts`.