不同贮藏温度对4种黄花菜花粉活力的影响

通过观察不同温度条件下黄花菜花粉的萌发情况,对4种黄花菜的花粉活力进行了测定。结果显示,室温条件下黄花菜花粉活力随贮藏天数的增加而迅速下降,3 d时活力下降至25%以下,难以满足杂交授粉中对花粉活力的要求;4,-20,-40℃条件下分别于10,20,30 d左右活力降至25%以下。说明室温条件下难以进行黄花菜花粉的贮藏,4℃适合小于10 d的短期贮藏,-20℃适合20 d以内的中期贮藏,-40℃适合60 d以内的长期贮藏;在相同贮藏温度下,大荔花和冲里花具有较高的花粉活力,适于杂交育种。

Influence of Different Storage Temperatures to the Pollen Viability of Four Hemerocallis citrine Baroni Varieties

In this study,the pollen viability of four Hemerocallis citrine Baroni varieties were tested under four different temperatures.The results showed：under the room-temperature,the pollen viability of Hemerocallis citrine Baroni decreased as the number of days increased,It decreased to 25％ on the 3rd day and failed to meet the requirement for the cross breeding.The pollen viability under 4,-20 and-40 ℃ decreased to 25％ respectively on the 10th,20th and 30th days.Conclusion is the pollen ofHemerocallis citrine Baroni is hard to be stored under the room-temperature,4 ℃ was suitable for shortterm storage of less than 10 days,-20 ℃ was suitable for mediumterm storage of less than 20 days,-40 ℃ was suitable for longterm storage of less than 60 days.At the same temperature,variety of Dali and Chongli had high pollen viability and suitable for cross breeding.