Identification and mapping of a quantitative trait locus controlling extreme late bolting in Chinese cabbage (Brassica rapa L. ssp. pekinensis syn. campestris L.) using bulked segregant analysis |
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Authors: | Hidetoshi Ajisaka Yasuhisa Kuginuki Susumu Yui Sueo Enomoto Masashi Hirai |
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Institution: | (1) National Research Institute of Vegetables, Ornamental Plants & Tea (NIVOT), Ano, Age, Mie 514-2392, Japan |
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Abstract: | DNA markers linked to a locus controlling an extreme late bolting trait, which was originally found in a local cultivar of
a non-heading leafy vegetable,‘Osaka Shirona Bansei’ (Brassica rapa L. ssp. pekinensis syn. campestris L.) were identified using bulked segregant analysis. A doubled haploid (DH) line, DH27, which is a progeny of ‘Osaka Shirona
Bansei’, shows extreme late bolting, and bolts without vernalization. DH27 was crossed with a normal bolting DH line, G309.
The plantlets of the parents, F1 and F2, were vernalized and then grown in a greenhouse. The bolting time of F2 plants showed a continuous distribution from 19 to 231 days after vernalization (DAV), suggesting the effects of a few major
genes and polygenes. Possible linkage markers for this trait were screened by modified bulked segregant analysis (BSA). The
BSA using four bulks suggested that a 530-bp RAPD band RA1255C was linked to a locus controlling the bolting trait. The RAPD
band was cloned and used as a probe to detect RFLP. The fragment detected a single locus, BN007-1,the segregation of which
in the F2 population matched that of RA1255C. Three other RAPDs were found to be linked to BN007-1. A quantitative trait locus(QTL)
affecting the bolting time was detected around BN007-1 using MAPMAKER/QTL. Since the difference between bolting times of both
the parental genotypes in the F2 was 138 days, these markers may be useful for a marker-assisted selection (MAS) in the breeding program for late bolting
or bolting-resistant cultivars in B. rapa crops.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Brassica rapa L syn campestris L bulked segregant analysis (BSA) DNA polymorphism flowering time late bolting quantitative trait locus (QTL) |
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