| 小菜蛾化学感受蛋白突变体的构建(英文) |
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| 引用本文： | 刘晶晶,胡美英,任珍珍,李亚楠. 小菜蛾化学感受蛋白突变体的构建(英文)[J]. 农业科学与技术, 2012, 0(1): 177-180 |
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| 作者姓名： | 刘晶晶 胡美英 任珍珍 李亚楠 |
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| 作者单位： | 华南农业大学昆虫毒理研究室 |
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| 基金项目： | Supported by Natural Science Foundation of Guangdong Province(9451064201003679);Guangdong Provincial Science and Technology Project(2009B020310005)~~ |
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| 摘 要： | [目的]研究半胱氨酸(Cys58)对小菜蛾(Plutella xylostella)化学感受蛋白1(PlxyCSP1)与农药化合物结合特性的影响。[方法]利用重叠延伸PCR法,将PlxyCSP1中Cys58突变成色氨酸(Trp58),获得突变体PlxyCSP1-M2。构建表达载体表达突变蛋白,并进行Western Blot检测。[结果]构建了突变基因的原核蛋白表达载体pET32a-PlxyCSP1-M2,并表达35kDa融合蛋白[结论]利用重叠延伸PCR法成功在原核系统中表达了PlxyC-SP1突变蛋白。
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| 关 键 词： | 小菜蛾 化学感受蛋白1 原核表达 |
A Mutant of PlxyCSP1 Protein in Plutella xylostella |
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| Jingjing LIU, Meiying HU, Zhenzhen REN, Yanan LI. A Mutant of PlxyCSP1 Protein in Plutella xylostella[J]. Agricultural Science ＆ Technology, 2012, 0(1): 177-180 |
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| Authors: | Jingjing LIU Meiying HU Zhenzhen REN Yanan LI |
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| Affiliation: | Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | [Objective] This study aimed to explore the effects of cysteine (Cys58) on the characteristics of PlxyCSP1 in P. lostella combined with pesticide compounds. [Method] Cys58 of PlxyCSP1 was mutated into Trp58 by using overlap extension PCR method, and PlxyCSP1-M2 mutant was obtained. Expression vector was constructed and the protein was detected by western blot. [Result] Expression vector pET32a-PlxyCSP1-M2 was constructed to express the 35kDa weight protein. [Conclusion] PlxyCSP1 mutant protein has been expressed successfully in prokaryotic expression system. |
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| Keywords: | P. xylostella PlxyCSP1 Prokaryotic expression system |
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