油松种子萌发过程中核酸和蛋白质的变化

随油松种子萌发的进程,种子内核酸总量、DNA,RNA和蛋白质含量发生不同程度的消长,胚蛋白组分的变化尤为明显,其中低等电点(pH4.3～5.2)区域的酸性蛋白质不断增加和累积;与此相反,高等电点(pH6.5～8.0)低分子量(4.3×10~4D以下)区域的碱性蛋白质逐渐降解、消失,用PAS反应证明它们是一组分子量各异的糖蛋白。种胚内的这些在不同时期出现和消失的蛋白质都具有萌发时期的特征性,它们可能对调节胚的生长和幼苗的形态建成有重要作用。

Changes of Nucleic Acids and Proteins in Germination of Chinese pine (Pinus tabulaefermis Carr.) Seed

Quantitative changes in the total amount of nucleic acids, DNA, RNA and protein contents in seeds of Chinese pine during germination were found. The most evident changes was in the components of embryonic proteins. Acid proteins with low isoelectric points (pH4.3-5.2) for example increased and accumulated continuously. On the contrary, basic proteins with high isoelectric points (pH6.5-8.0) , and low moleculer weight (<4.3×104D) gradually degraded and disappeared. As proved with P.A.S. reaction, they are a group of glycoproteins of different molecular weights. These proteins in the embryo of Chinese pine seeds are characterized by their distinct appearance and disappearance at specific stages of germination. This finding could have far reaching importance in regulating the growth of embryos and the morphogenesis of seedlings.