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| 单核苷酸多态性在大豆育种中的应用 | |
| 引用本文： | 许阳,李东潮,李晓丽,王飞雪,徐青,张明辉. 单核苷酸多态性在大豆育种中的应用[J]. 安徽农业科学, 2004, 32(5): 1000-1002 |
| 作者姓名： | 许阳 李东潮 李晓丽 王飞雪 徐青 张明辉 |
| 作者单位： | 1. 河南省南阳市农业科学研究所,河南南阳,473000 2. 河南省博爱农场种子公司 3. 河南省邓州市种子公司 |
| 摘 要： | 单核苷酸多态性 (singlenucleotidepolymorphism ,SNP)是指DNA序列上的单个碱基变异 ,它具有分布广、多态信息量大、易于检测和统计分析等优点 ,被称为继RFLP和微卫星标记后的第三代基因遗传标记。单核苷酸多态性是等位基因间序列差异最为普遍的类型 ,可作为一种高通量的遗传标记。已建立PCR扩增目标序列及其产物测序和电子SNP(eSNP)等多种发现和检测SNP的方法。大豆等作物也已开展了SNP分析。 |
| 关 键 词： | 单核苷酸多态性(SNPs) 分子标记 大豆 连锁不平衡 |
| 文章编号： | 0517-6611(2004)05-1000-03 |
Application of Single Nucleotide Polymorphism in Soybean |
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| Xu Yang et al. Application of Single Nucleotide Polymorphism in Soybean[J]. Journal of Anhui Agricultural Sciences, 2004, 32(5): 1000-1002 | |
| Authors: | Xu Yang et al |
| Abstract: | Single nucleotide polymorphism (SNP) refers to the change of single nucleotide in DNA sequence. Because of its high density in genomes and easy in detection and analysis statistically, SNP can be used in genetic linkage map construction and QTL mapping. Single nucleotide polymorphism( SNP) is the most common type of sequence difference between alleles,which can be used as a kind of high-throughput genetic marker. Several different routes were developed to discover and identify SNP. These included the direct sequencing of PCR amplicons, electronic SNP(eSNP) and so on. SNP assays were made in many crop species such as soybean. SNP analysis was broadly used in the field of plant gene mapping, integration of genetic and physical maps, DNA marker-assisted breeding and functional genomics. |
| Keywords: | Single nucleotide polymorphism(SNP) Soybean molecular marker |
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