Vegetative propagation of the threatened African wild olive [Olea europaea L. subsp. cuspidata (Wall. ex DC.) Ciffieri] |
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Authors: | Negash Legesse |
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Affiliation: | (1) Department of Biology, Faculty of Science, Addis Ababa University, Addis Ababa, Ethiopia (e-mail |
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Abstract: | A rapid vegetative propagation method is described for Olea europaea L. subsp. cuspidata [Wall. ex DC.) Ciffieri (syn. Oleaafricana Mill.)]. Leafy branch cuttings were harvested from 6 to 7-month-old stecklings (= plants derived from rooted cuttings). Cuttings 2–3 mm in basal diameter were trimmed to 70–100 mm in length, with leaf areas reduced to 15–33 cm2. Indolebutyric acid (IBA) was applied at 0, 10, 20, 25, 30, 35 and 40 µg/cutting to each of the 70–80 replicate cuttings randomly allocated to each of the 7 treatments. Callusing and rooting occurred 3–5 and 5–7 weeks after treatment, respectively. The rooting success of cuttings treated with 20 or 40 µg IBA/cutting was 75 and 90%, respectively. Differences in rooting success, speed of rooting and root numbers between the control and the IBA treatments were significant (p < 0.01). Compared to seedlings of similar shoot height, rooted cuttings grew significantly (p < 0.01) faster and produced more biomass. It is concluded from this study that the rooting of leafy branch cuttings derived from young stockplants of wild olive is rapid, and that vegetative propagation is an effective means of regenerating this valuable, yet threatened, tree species. |
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Keywords: | Adventitious rooting Callusing Indolebutyric acid Leafy branch cuttings Propagator |
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