| 猪链球菌2型江苏分离株溶血素基因检测及序列分析 |
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| 引用本文： | 方绍庆 陆承平. 猪链球菌2型江苏分离株溶血素基因检测及序列分析[J]. 中国动物检疫, 2005, 22(11): 18-20 |
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| 作者姓名： | 方绍庆 陆承平 |
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| 作者单位： | 南京农业大学农业部动物疫病诊断重点开放实验室,210095 |
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| 基金项目： | 国家973项目子课题（G1999011906） |
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| 摘 要： | 根据猪链球菌2型（Streptococcus suis type 2）溶血素基因（sly）设计和合成了一对可扩增其完整阅读框的引物，对HA9801等6株猪链球菌2型江苏分离株，1株德国分离株SS2-D及猪链球菌C群参考株ATCC35246的核酸进行PCR扩增，结果显示HA9801等6株江苏分离株及德国株SS2呈阳性．ATCC35246呈阴性。HA9801株PCR产物纯化后测序．序列分析表明该DNA片段与猪链球菌2型1933株的sly基因同源性为99％。
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| 关 键 词： | 猪链球菌2型 溶血素 核苷酸序列 溶血素基因 序列分析 分离株 基因检测 江苏 PCR扩增 基因同源性 |
| 文章编号： | 1005-944X(2005)11-0018-03 |
Detection and sequence analysis of the gene encoding suilysin from Streptcoccus suis type 2 strains isolated in Jiangsu province |
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| Fang Shaoqing,Lu Chengping. Detection and sequence analysis of the gene encoding suilysin from Streptcoccus suis type 2 strains isolated in Jiangsu province[J]. China Animal Health Inspection, 2005, 22(11): 18-20 |
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| Authors: | Fang Shaoqing Lu Chengping |
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| Affiliation: | Yantai Entry-Exit Inspection and Quarantine Bureau |
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| Abstract: | A hemolysin gene from six strains of Streptcoccus suis type 2 isolated in Jiangsu was amplified by PCR using a pair of specific primers.The PCR product from strain HA9801 was sequenced and nucleotide sequence analysis revealed the hemolysin gene from strain HA9801 had a high homology to strains 1933(99%). |
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| Keywords: | Streptcoccus suis type 2 Suilysin Nucleotide sequence |
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