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Gene expression profile of IGF1 and MSTN mRNA and their correlation with carcass traits in different breeds of geese at 70 d of age |
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| Authors: | Q Tang C Song S Zhang Y Hu D Zhao |
| Institution: | 1. Key Laboratory of Poultry Heredity &2. Breeding, Jiangsu Institute of Poultry Science, Yangzhou 225125, China |
| Abstract: | 1. The expression of insulin-like growth factor-1 (IGF1) and myostatin (MSTN) mRNA in breast and leg muscle was quantified in 70-d-old Taihu and Wanxi geese by using a Multiplex Competitive Fluorescent–PCR method and the correlations between mRNA levels and carcass traits were analysed. 2. IGF1 mRNA expression in breast muscle in Taihu geese was significantly higher than that in Wanxi geese and the MSTN mRNA level in leg muscle in Taihu geese was significantly higher than that in Wanxi geese. 3. There was no significant difference in breast muscle MSTN or leg muscle IGF1 mRNA expression between the two breeds. 4. Within the same breed, the IGF1 mRNA expression in leg muscle of male geese was significantly higher than that in female geese, and MSTN mRNA expression in leg muscle was significantly higher than that in breast muscle. 5. There was no difference in the IGF1 mRNA expression between tissues. 6. There was a positive correlation between IGF1 mRNA and MSTN mRNA and a negative correlation between IGF1 mRNA expression of breast muscle and leg muscle ratio. 7. In Wanxi geese, MSTN mRNA expression in leg muscle was negatively associated with body weight and leg muscle weight. |
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