| 鹿角托盘总蛋白的提取工艺研究 |
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| 引用本文： | 黄萍,赵雨,牛放,杨菲,张连学. 鹿角托盘总蛋白的提取工艺研究[J]. 安徽农业科学, 2010, 38(14): 7305-7307. DOI: 10.3969/j.issn.0517-6611.2010.14.062 |
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| 作者姓名： | 黄萍 赵雨 牛放 杨菲 张连学 |
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| 作者单位： | 吉林农业大学中药材学院,吉林长春,130118;长春中医药大学中医药与生物工程研发中心,吉林长春,130117 |
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| 基金项目： | 国家"十一五"科技支撑计划项目 |
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| 摘 要： | [目的]优化鹿角托盘总蛋白提取工艺。[方法]以总蛋白得率为评价指标,采用单因素试验和正交试验设计优选最佳提取工艺。[结果]最佳提取工艺为：用含0.3mol/LNaCl,20mmol/LNa2HPO4缓冲液（pH值为9）提取,料液比为1：15,提取时间为24h,提取3次。在此工艺条件下,总蛋白得率达10%以上。[结论]试验优选出的工艺稳定、合理、可行。
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| 关 键 词： | 鹿角托盘 总蛋白 提取工艺 正交试验 |
Studies on the Extraction Process of Total Proteins from Sika Antlerbase |
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| HUANG Ping et al. Studies on the Extraction Process of Total Proteins from Sika Antlerbase[J]. Journal of Anhui Agricultural Sciences, 2010, 38(14): 7305-7307. DOI: 10.3969/j.issn.0517-6611.2010.14.062 |
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| Authors: | HUANG Ping et al |
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| Affiliation: | HUANG Ping et al (College of Traditional Chinese Medicine Materials,Jilin Agricultural University,Changchun,Jilin 130118) |
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| Abstract: | [Objective]To optimize the extraction process of total protein from Sika antlerbase. [Method]Through using total protein yield as the evaluation index,the optimum extraction conditions were determined by single-factor and orthogonal design. [Result]The optimum extraction conditions were as follows:containing 0. 3 mol/L NaCl,20 mmol/L of Na2HPO4 buffer solution (pH 9),extraction time for 24 h,solid-liquid ratio of 1∶15 and extracting for three times. The total protein yield was up to 11% or more. [Conclusion]The optimized process is stable,reasonable and feasible. |
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| Keywords: | Sika antlerbase Total proteins Extraction process Orthogonal test |
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