| 大肠杆菌SerB基因的克隆及其在黄色短杆菌中的表达 |
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| 引用本文： | 殷丽霞,崔春生,简子健,谭慧林,包慧芳. 大肠杆菌SerB基因的克隆及其在黄色短杆菌中的表达[J]. 新疆农业科学, 2006, 43(2): 96-99 |
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| 作者姓名： | 殷丽霞 崔春生 简子健 谭慧林 包慧芳 |
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| 作者单位： | 新疆农业大学动物医学学院,新疆乌鲁木齐,830052;新疆农科院微生物所,新疆乌鲁木齐,830000 |
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| 摘 要： | 磷酸丝氨酸转氨酶(Phosphoserine aminotransferase,SerB)是L-丝氨酸生物合成中的关键酶,应用PCR技术从大肠杆菌JM109(E.Coli JM109)中扩增出磷酸丝氨酸转氨酶基因,将其与表达载体pEC 7连接.通过电转化方法,将重组质粒转入黄色短杆菌(Brevibacterium flavum C-11,BfC-11)中,经酶活检测和摇瓶发酵培养,含有重组表达质粒的BfC-11B的磷酸丝氨酸转氨酶的活力和L-丝氨酸产率均比原宿主菌BfC-11有所提高,为构建L-丝氨酸高产基因工程菌的研究奠定了基础.
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| 关 键 词： | 磷酸丝氨酸转氨酶 克隆 表达 |
| 文章编号： | 1001-4330（2006）02-0096-04 |
| 收稿时间： | 2005-12-13 |
| 修稿时间： | 2005-12-13 |
Cloning of Phosphoserine Aminotransferase (SerB) Gene of Escherichia coli and Expression in Brevibacterium flavum |
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| YIN Li-xia,CUI Chun-sheng,JIAN Zi-jian,TAN Hui-lin,BAO Hui-fang. Cloning of Phosphoserine Aminotransferase (SerB) Gene of Escherichia coli and Expression in Brevibacterium flavum[J]. Xinjiang Agricultural Sciences, 2006, 43(2): 96-99 |
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| Authors: | YIN Li-xia CUI Chun-sheng JIAN Zi-jian TAN Hui-lin BAO Hui-fang |
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| Affiliation: | 1. College of Animal Medicine, Xinjiang Agricultural University, Urumqi 830052, Cina; 2. Insitute of Microbiology, Xinjang Academy of Agricultural Science, Urumqi 830000, Cina |
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| Abstract: | Phosphoserine aminotransferase(SerB) is a key enzyme in biosynthesis of L-serine.The SerB gene is obtained from E.coli JM109 by PCR. This gene was inserted into pEC7 and the recombinant plasmids with SerB gene were transformed into Brevibacterium flavum C-11(BfC-11)by the method of electrotransformation and by detecting enzyme activity and the fermentation culture of BfC-11 and BfC-11B.The results showed that the activity of SerB and the yield of L-serine in BfC11B are higher than those in BfC-11.It lays foundations for the research of construction of high L-serine produced gene-engineering strain. |
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| Keywords: | Phosphoserine aminotransferase cloning expression |
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