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| 不同培养基组分对5个蜡梅品系花粉萌发和花粉管生长的影响 | |
| 引用本文： | 沈植国,孙萌,丁鑫,程建明,陈迪新.不同培养基组分对5个蜡梅品系花粉萌发和花粉管生长的影响[J].浙江农业学报,2021,33(2):278-287. |
| 作者姓名： | 沈植国 孙萌 丁鑫 程建明 陈迪新 |
| 作者单位： | 1.中南林业科技大学 林学院,湖南 长沙 4100042.河南省林业科学研究院,河南 郑州 4500083.河南科技大学 园艺与植物保护学院,河南 洛阳 471023 |
| 基金项目： | 河南省科技攻关项目(182102110458);河南省科技兴林项目(豫林计〔2018〕127号);河南省基本业务费(2018JB02-001) |
| 摘 要： | 以河南省许昌市3个类型5个品系的蜡梅花粉为试验材料,研究了不同培养基组分对蜡梅花粉萌发和花粉管生长的影响,以筛选出适宜不同类型蜡梅花粉萌发的最佳培养基,为生产实践中科学授粉和杂交育种提供理论依据.结果表明:聚乙二醇是蜡梅花粉萌发和花粉管生长的关键因素,当培养基中没有聚乙二醇时,花粉不能萌发;蔗糖不是蜡梅花粉离体萌发的必... |
| 关 键 词： | 蜡梅 花粉活力 培养基组分 花粉萌发 花粉管长度 |
| 收稿时间： | 2020-05-13 |
Effects of different culture medium composition on pollen germination and pollen tube growth of 5 strains of Chimonanthus praecox |
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| SHEN Zhiguo,SUN Meng,DING Xin,CHENG Jianming,CHEN Dixin.Effects of different culture medium composition on pollen germination and pollen tube growth of 5 strains of Chimonanthus praecox[J].Acta Agriculturae Zhejiangensis,2021,33(2):278-287. | |
| Authors: | SHEN Zhiguo SUN Meng DING Xin CHENG Jianming CHEN Dixin |
| Institution: | 1. College of Forestry, Central South University of Forestry and Technology, Changsha 410004, China 2. Henan Academy of Forestry, Zhengzhou 450008, China 3. College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China |
| Abstract: | Five strains of Chimonanthus praecox fresh pollens collected from Xuchang City, Henan Province were used as materials to test and screen the best culture medium for pollen germination of C. praecox. Effects of different culture medium composition on pollen germination and pollen tube growth of C. praecox were investigated with in-vitro culture method. The results showed that PEG-4000 was the key factor for pollen in-vitro germination and its tube growth of C. praecox, and the pollen failed to germinate when there was no PEG-4000 in the medium. On the contrary, sucrose was unnecessary factor for pollen in-vitro germination and high concentration sucrose inhibited pollen germination; While boric acid and calcium nitrate could promote pollen germination effectively, which played an auxiliary role in pollen germination. The optimum medium formulation for C. praecox var. concolor and C. praecox var. patens was 250 g·L-1 PEG-4000+10 mg·L-1 Ca (NO3)2·4H2O+20 mg·L-1 H3BO3, the pollen germination rates of Yusu No.1, Yusu No.2 and Yuhong No.1 were 25.38%, 25.34% and 23.46%, respectively, and their pollen tube lengths were 302.31 μm, 297.64 μm and 285.68 μm, respectively. The optimum medium formulation for C. praecox var. intermedius was 250 g·L-1 PEG-4000+5 mg·L-1 Ca(NO3)2·4H2O+15 mg·L-1 H3BO3, the pollen germination rates of Yuqiao No.1 and Yuqiao No.2 were 24.43% and 24.93%, their pollen tube lengths were 288.27 μm and 286.37 μm, respectively. The influence trend of the same factors in the culture medium was the same for the five kinds of pollen, but under the most suitable conditions, the pollen vitality of C. praecox var. concolor was better, and its germination rate was slightly higher than that of the other two types. |
| Keywords: | Chimonanthus praecox pollen viability culture medium composition pollen germination pollen tube length |
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