浙江省亚洲柑橘木虱黄龙病病原分布与消长规律

亚洲柑橘木虱Diaphorina citri Kuwayama是黄龙病病原Candidatus Liberibacter asiaticus(CLas)的主要传播媒介.为更好地探清亚洲柑橘木虱上的CLas阳性分布特性及其动态变化,提出适宜治虫防病的窗口期,特针对浙江省柑橘果园的分布特点,于2002—2019年采取定园定...

Distribution and growth dynamics of Candidatus Liberibacter asiaticus positive Asian citrus psyllid in Zhejiang Province

Asian citrus psyllid (Diaphorina citri Kuwayama) is the main vector of Candidatus Liberibacter asiaticus(CLas), the pathogen of Huanglongbing (HLB) in China. In order to reveal the distribution characteristics and growth dynamics of CLas in Asian citrus psyllid, and propose suitable time for pest control and disease prevention, 1 662 samples of Asian citrus psyllid and 2 900 samples of HLB were collected from orchards in 24 counties (cities and districts) from 2002 to 2019, according to the distribution characteristics of citrus orchards in Zhejiang Province. The quantity and positive rate of CLas were detected by quantitative PCR. It was shown that there was linear relationship within CLas amount and latitude, longitude, respectively. It was calculated that the north and west boundary for CLas distribution was 29.20°N and 119.80°E in Zhejiang Province. The positive rate of CLas in Zhejiang orchards increased with the elevated HLB incidence rate, and their relationship fitted the Logistic model. The monthly dynamics of CLas in Zhejiang orchards exhibited 3 peaks in April, July and December, respectively, while the annual dynamics fitted conic curves, and reached the peak in 2010. Based on these findings, it was suggested that early spring, summer and autumn were the best time for HLB prevention and control.