贵州地方中国樱桃资源的ISSR分析

利用12条ISSR引物对40种樱桃资源进行遗传多样性分析,结果显示,总位点数为120,平均观测等位基因数为1.75,平均有效等位基因数为1.30,平均Nei’s遗传多样性为0.19,平均香浓信息指数为0.30。毕节地区遗传多样性最高,且野生资源遗传多样性高于栽培资源。聚类结果显示,相似性系数在0.64~0.98之间,以0.81为阈值,可以把野生资源和栽培资源区分开,其中栽培种中可以分为3个亚组。玛瑙红樱桃与雷公山两种野生樱桃聚在一起,猜测有野生樱桃血统,黔西“红皮”与“青皮”樱桃聚在一起,遗传相似性最高,与其他所有樱桃亲缘关系较远。

ISSR Analysis of Local Chinese Cherry Germplasm Resources in Guizhou

In this paper,genetic diversity of 40 Prunus pseudocerasus germpalms were analyzed using 12 ISSR primers.The results showed that the total number of loci was 120,and the average number of observed alleles was 1.75.In addition,the average number of effective alleles and the average Nei’s genetic diversity were 1.30 and 0.19 respectively,and the average Shannon information index was 0.30.The genetic diversity of wild P.pseudocerasus germpalms was higher than that of cultivated resources in Bijie region.The clustering results showed that the similarity coefficient ranged from 0.64 to 0.98,and 0.81 was used as the threshold value to distinguish wild resources from cultivated resources.Further,The cultivated species could be divided into three subgroups.Two kinds of wild P.pseudocerasus,Manaohong and Leigongshan,were gathered together which implied both of them possessed wild P.pseudocerasus pedigree.Additionally,the other two varieties,Hongpi and Qingpi from Qianxi region of Guizhu province,were clustered together with the highest genetic similarity,and had a distant relationship with all other varieties.