新疆寒冷地区腐木中产纤维素酶菌株的筛选与低温产酶特性

为筛选出能在低温条件下高效降解纤维素的菌株,提高秸秆在低温条件下纤维素的降解速度,以新疆寒冷地区腐木为试验材料,对低温纤维素降解菌进行筛选,在4℃条件下筛选得到4株可在低温下生长且具有纤维素降解作用的真菌,通过形态学和分子生物学的方法对低温菌进行鉴定,分别为产黄青霉(Penicilli-um chrysogenum)、...

Screening of cellulase producing strains from rotten wood in Xinjiang cold area and analysis of their characteristics of enzyme production at low temperature

In order to screen out the strains which can degrade cellulose efficiently at low temperature and improve the degradation rate of cellulose under low temperature, in this study, rotten wood in cold area of Xinjiang was used as experimental material, and the low-temperature cellulose degrading bacteria were screened. Four strains of fungi which could grow at low temperature and degrade cellulose were screened at 4 ℃. Morphological and molecular biological methods were used for identification of low temperature strains. They were Penicillium chrysogenum, two Trichoderma citrinoviride strains and Neurospora sitophila. The cold tolerance test showed that all the strains were cold resistant. Studies on the characteristics of enzyme production of 4 low-temperature bacteria showed that the optimal culture time for cellulase production was 9 d, the optimal initial pH of the culture medium was 7, the optimal temperature was 25 ℃, and the optimal inoculation amount was 5%. The straw degradation test showed that these four strains of fungi had ability to degrade the straw, and had the best degradation effect on the corn straw with the fermentation rate above 40%.