高粱种质资源出苗相关性状与SSR标记的关联分析

选用54对均匀分布在高粱全基因组的SSR标记对110份高粱种质资源进行检测,研究高粱中胚轴长、胚芽鞘长、根长和百粒重4个出苗相关性状与SSR标记的关联性,以期为选育耐深播的优异高粱资源提供理论支持和材料保障.结果表明,高粱中胚轴长与根长呈显著(P≤0.05)负相关,与胚芽鞘长呈极显著(P≤0.01)正相关.胚芽鞘长、根...

Association analysis between SSR markers and germination-related traits in sorghum germplasm resources

Fifty-four pairs of simple sequence repeat (SSR) markers spanned uniformly over sorghum genome were selected to detect 110 sorghum germplasm resources. The relevances between SSR and germination-related traits (mesocotyl length, coleoptile elongate, root length, and hundred-grain weight) were studied in order to provide theoretical foundation and materials for screening elite deep-seeding tolerance in sorghum. The results showed that there were significantly (P≤0.05) negative correlation between mesocotyl length and root length, and significantly (P≤0.01) positive correlation between mesocotyl length and coleoptile length. Significantly (P≤0.01) positive correlations were found among coleoptile length, root length, and hundred-grain weight. A total of 339 alleles were identified at all of 54 SSR markers loci, with an average of 6.28 alleles per marker locus and 2.67 effective alleles. The average values of Shannon’s information index were 1.129 9. All of the sorghum germplasms were divided into three subgroups by structural analysis. Nine sites had significant (P≤0.05) correlation with mesocotyl length, whose phenotypic contribution rate was from 11.18%-28.05%. Five sites had significant (P≤0.05) correlation with coleoptile elongate, whose phenotypic contribution rate were 11.22%-18.12%. Only one site had significant (P≤0.05) correlation with root length. Two sites had significant correlation with hundred-grain weight.