口服三七总皂甙对大黄鱼免疫功能的影响

将含不同浓度三七总皂甙(PNS)的饲料(0、10、50、250 mg·kg-1)连续14 d投喂大黄鱼,检测肝、脾、头肾组织部分免疫相关基因的表达,以及血清部分免疫因子的活性,结合人工注射感染变形假单胞菌的抵抗力,评估口服PNS对大黄鱼免疫功能的影响.实验结果显示,各浓度组大黄鱼血清超氧化物歧化酶(SOD)、溶菌酶(L...

Effects of oral Panax notoginseng saponins on immunity of large yellow croaker (Pseudosciaena crocea)

In this study, large yellow croaker (Pseudosciaena crocea) were fed with diets containing 0,10,50,250 mg·kg^-1 Panax notoginseng saponins (PNS) for 14 d. To evaluate the effects of PNS on the immunity of large yellow croaker, the expression of some immune-related genes in the liver, spleen and head kidney tissues, the activities of some serum immune factors and the resistance against Pseudomonas plecoglossicida were determined. Results showed that the activities of serum superoxide dismutase (SOD), lysozyme (LZM), acp acid phosphatase (ACP) and alkaline phosphatase (AKP) in the experimental groups were not significantly different from the control group; the expression of Mx in spleen of fish fed with 250 mg·kg ^-1 PNS was significantly higher than that of control; the expression of Mx in spleen of fish fed with 50 mg·kg ^-1 PNS and in head-kidney of fish fed with 10 mg·kg^-1 PNS were higher than that of control, but not significantly; the expression of TNF-α in all the exprimental groups were lower than that of control, but not significantly; the expression of IL-10 and MHCⅡ-α in all the exprimental groups were not significantly different from the control. The mortalities of the exprimental fish challenged with P. plecoglossicida were not significantly different from the control. It was indicated that feeding diets containing 10-250 mg·kg ^-1 PNS for 14 d did not improve the immunity of large yellow croaker