杜仲ABC转运蛋白基因家族成员鉴定与分析

ABC转运蛋白(ATP—binding cassette transpoter)是一类庞大而古老的跨膜运输蛋白家族,在生物体内参与多种物质的转运积累、有害物质解毒、气孔调节、植物防御等生理活动。杜仲(Eucommia ulmoides Oliv)作为重要的中药材,其药用成分主要为次生代谢产物,次生代谢物转运与积累过程需要ABC转运蛋白的参与。利用生物信息学手段对杜仲ABC转运蛋白基因家族进行鉴定,并分析该家族蛋白质性质和结构、跨膜结构、亚细胞定位、系统进化关系。研究表明,EuABC家族生物信息学预测有76个成员,含有1~7个保守基序;编码蛋白多为稳定蛋白,主要分布于细胞质膜上,二级结构以α-螺旋和无规卷曲为主要构成元件;进化树分析表明,杜仲ABC转运蛋白家族可分为8个亚家族(A~G;I),每组成员数量分别为3、19、14、1、1、1、29、8。研究结果可为进一步研究杜仲次生代谢物质转运与积累奠定基础,也为其他植物ABC转运蛋白家族的研究提供了参考依据。

Identification and analysis of ABC transporters family from Eucommia ulmoides

ATP-binding cassette (ABC) transpoter is ancient and huge family of transmembrane transport proteins, which are involved in the transport and accumulation of a variety of substances, detoxification of harmful substances, stomatal regulation, plant defense and other physiological activities in organisms. Eucommia ulmoides Oliv, as an important Chinese medicinal material, is mainly composed of secondary metabolites, and the transport and accumulation of secondary metabolites require the participation of ABC transporters. In this study, ABC transporter gene family in E. ulmoides was identified, then its molecular characteristics were analyzed from the aspects of gene identification, protein properties, protein structure, transmembrane structure, subcellular localization and phylogenetic relationship by bioinformatics method. The results predicted by bioinformatics showed that EuABC transporter protein family had 76 members, including 1-7 conserved motifs; most of the encoded proteins were stable proteins, which were mainly distributed on the plasma membrane, and the main component of secondary structure was α-helix and random coil, mainly distributed in the plasma membrane. Analysis of evolutionary tree showed that EuABC transporter family could be divided into eight subfamilies (A-G and I), and the number of members in each group was 3, 19, 14, 1, 1, 1, 29 and 8, respectively. The research results provided a favorable foundation for further study on the transport and accumulation of secondary metabolites in E. ulmoides, and also provided a reference for study of ABC transporter families of other plant.