白头翁皂苷B4对临床型奶牛乳房炎疗效和血清炎性因子、免疫因子的影响

为观察不同剂量白头翁皂苷B4对临床型奶牛乳房炎的疗效,及对血清炎性因子和免疫因子的影响,将36头患临床型乳房炎的奶牛随机分为低、中、高剂量3个试验组,同时选择12头健康奶牛作为对照组.试验组每日分别于颈部肌肉注射白头翁皂苷B40.025、0.05、0.1 mL·kg-1,连续注射6 d,对照组不注射任何药物.试验期间,...

Effect of Pulsatilla saponin B4 on treatment efficiency and serum inflammatory and immune factors of dairy cows with clinical mastitis

To investigate the effects of Pulsatilla saponin B4 on therapeutic effect, serum inflammatory and immune factors in dairy cows with clinical mastitis, 12 healthy dairy cows were selected as the control group, and 36 clinical mastitis cows were selected as the test group and were randomly divided into low, middle and high dose group. Cows in test group were administered with Pulsatilla saponin B4 injection (with doses of 0.025, 0.05, 0.1 mL·kg^-1, respectively) through cervical intramuscular injection once a day for 6 days, whereas no medicine was administered in the control group. The effective and cure rates of cows in the test group were recorded, venous blood samples were collected (any day in the control group and the 1^st, 3^rd, 5^th, 7^th day in the test group) to measure serum inflammatory and immune factors, as well as milk in the infected quarters on the 1^st, 7^th, 12^th day in the test group to conduct somatic cells count (SCC) and pathogenic microorganism isolation and identification during this experiment. Cows were observed till the 12^th day and the therapeutic efficiency was recorded. It was shown that the clinical cure rate and effective rates of low, middle and high dose group were 75.00%, 75.00%, 91.67% and 100%, 100%, 100%, respectively, and the average cure time was 6.33, 6.11, 6.91 d, respectively. The Pulsatilla saponin B4 could significantly (P<0.05) attenuate SCC. Before treatment, 34 out of 36 milk samples were positive with pathogenic microorganism, indicating a detection rate of 94.44%, which mainly consisted of mycoplasma, cocci and Escherichia coli. After treatment, the total negative rate was 52.94%, among which, the negative rate of Bacilli, Staphylococcus chromogenes, Staphylococcus haemolyticus and Escherichia coli were 100%, while the negative rates of mycoplasma, Staphylococcus aureus, Staphylococcus epidermidis and coccus were 69.23%, 60.00%, 50.00% and 33.33%, respectively. Besides, Pulsatilla saponin B4 could significantly (P<0.05) promote the recovery of inflammatory factors CRP, SAA, HP, IL-1α, IL-1β, IL-2, IL-6, IL-8, TNF-α and immune factors IgA, IgD, IgE, IgG, IgM to normal levels.