| 叶底红组织培养和快速繁殖条件的优化 |
| |
| 引用本文： | 蔡坤秀,陈振东,林宗铿,林秀香,郑少缘.叶底红组织培养和快速繁殖条件的优化[J].福建农业学报,2012(6):621-625. |
| |
| 作者姓名： | 蔡坤秀 陈振东 林宗铿 林秀香 郑少缘 |
| |
| 作者单位： | 福建省热带作物科学研究所 |
| |
| 基金项目： | 福建省科技计划重点项目(2004N012) |
| |
| 摘 要： | 应用正交试验方法研究6-BA,NAA及KT等3个因子对叶底红组培苗增殖系数的影响,同时考查了IBA、NAA和活性炭等3个因子对叶底红组培苗生根培养的影响,试验均按正交表L9(34)形成三因素、三水平的不同配比。结果表明:适宜叶底红增殖的培养基为MS+6-BA 1.2mg.L-1+NAA 0.4mg.L-1+KT 0.1mg.L-1,平均增殖系数3.62;适于生根的培养基为1/2MS+IBA 0.5mg.L-1+活性炭1.0g.L-1。以泥炭土和珍珠岩(2∶1)作为移栽基质,移栽成活率可达90%以上。
|
| 关 键 词： | 叶底红 组织培养 快速繁殖 正交试验 |
Optimization of Culture and Rapid Propagation for Phyllagathis fordii |
| |
| CAI Kun-xiu,CHEN Zhen-dong,LIN Zong-keng,LIN Xiu-xiang,ZHENG Shao-yuan.Optimization of Culture and Rapid Propagation for Phyllagathis fordii[J].Fujian Journal of Agricultural Sciences,2012(6):621-625. |
| |
| Authors: | CAI Kun-xiu CHEN Zhen-dong LIN Zong-keng LIN Xiu-xiang ZHENG Shao-yuan |
| |
| Institution: | (Fujian Institute of Tropical Crops,Zhangzhou,Fujian 363001,China) |
| |
| Abstract: | Effects of hormones and activated carbon in medium on the proliferation and rooting of Phyllagathis fordii were studied for the culture optimization.Plant tissues from aseptic seedlings were used as the explants for the orthogonal design experiment L9(34)].The result showed that(a) MS supplemented with 6-BA 1.2 mg·L-1+NAA 0.4 mg·L-1+KT 0.1 mg·L-1 was appropriate for the proliferation;(b) 1/2 MS supplemented with IBA 0.5 mg·L-1+activated carbon 1.0 g·L-1 was suitable for rooting;and(c) the survival rate were greater than 90% when the plantlets were transplanted to a soil medium containing 2/3 peat and 1/3 perlite. |
| |
| Keywords: | Phyllagathis fordii tissue culture rapid propagation orthogonal experiment |
| 本文献已被 CNKI 等数据库收录！ |
|
