| 一个与水稻直立穗基因qPE9-1关联的InDel标记的设计与验证 |
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| 引用本文： | 朱金燕,王军,杨杰,范方军,杨金欢,仲维功. 一个与水稻直立穗基因qPE9-1关联的InDel标记的设计与验证[J]. 分子植物育种, 2012, 0(5): 583-588 |
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| 作者姓名： | 朱金燕 王军 杨杰 范方军 杨金欢 仲维功 |
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| 作者单位： | 江苏省农业科学院粮食作物研究所,江苏省优质水稻工程技术研究中心,国家水稻改良中心南京分中心;安徽农业大学生命科学院 |
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| 基金项目： | 国家与江苏省粮食丰产科技工程(2011BAD16B03/BE2009425);江苏省农业科技自主创新基金(CX[11]4020)共同资助 |
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| 摘 要： | 水稻穗型是重要的农艺性状,也是水稻高产的重要决定因素之一。本研究根据水稻弯曲穗品种与直立穗品种在直立穗基因qpe9-1第5外显子处存在的核苷酸差异设计了1对插入-缺失标记(Insert/Delection),InDel-E5。利用该功能标记对24份水稻品种资源材料及直立穗分离群体F2进行标记基因型分析,研究结果表明,直立穗品种均表现为缺失带型,弯曲穗品种均表现为非缺失带型,F2群体中杂合带型和非缺失带型均为弯曲穗,而缺失带型均为直立穗,说明利用该标记可以快速、准确的鉴定出直立穗品种和单株。
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| 关 键 词： | 水稻 直立穗 弯曲穗 功能标记 |
Development and Application for a Functional Marker for Erect Panicle Gene qPE9-1 of Rice |
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| Zhu Jinyan,Wang Jun,Yang Jie,Fan Fangjun,Yang Jinhuan,ZhongWeigong. Development and Application for a Functional Marker for Erect Panicle Gene qPE9-1 of Rice[J]. Molecular Plant Breeding, 2012, 0(5): 583-588 |
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| Authors: | Zhu Jinyan Wang Jun Yang Jie Fan Fangjun Yang Jinhuan ZhongWeigong |
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| Affiliation: | 1* 1 Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, 210014; 2 College of Life Sciences, Anhui Agricultural University, Hefei, 230036 |
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| Abstract: | Rice plant architecture is an important agronomic trait and a major determinant in high productivity. In this study, one InDel marker, InDel-E5, was designed based on the difference of extron 5 between curved panicle gene qPE9-1 and erect panicle qpe9-1. 24 rice varieties and F2 segregated populations were detected for genotype analysis using InDel-E5 marker. The results showed that InDel-E5 was cosegregated with the rice panicle erectness, allowing to identify the homozygous erect panicle genotype, homozygous curved panicle genotype and heterozygous curved panicle genotype individuals. Therefore, InDel-E5 can be used to determined rice panicle genotype rapidly and exactly. |
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| Keywords: | Rice Erect panicle Curved panicle Functional marker |
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