| 家蚕幼虫消化液蛋白质组学分析 |
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| 引用本文： | 崔颖俊,裘智勇,赵巧玲,沈兴家,唐顺明,郭锡杰.家蚕幼虫消化液蛋白质组学分析[J].蚕业科学,2008,34(1):50-53. |
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| 作者姓名： | 崔颖俊 裘智勇 赵巧玲 沈兴家 唐顺明 郭锡杰 |
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| 作者单位： | 江苏科技大学生物与环境工程学院,江苏镇江,212018;中国农业科学院蚕业研究所,农业部家蚕生物技术重点开放实验室,江苏镇江,212018;中国农业科学院蚕业研究所,农业部家蚕生物技术重点开放实验室,江苏镇江,212018 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 为了研究家蚕幼虫消化液的蛋白质组成情况,用双向电泳(2-DE)技术对家蚕幼虫消化液中的蛋白质进行了分离,随后用基质辅助激光解析电离飞行时间质谱(MALD I-TOF MS)对其中的8个高丰度蛋白进行了鉴定,并用生物信息学方法进行了分析。结果显示:家蚕幼虫消化液中的蛋白质种类较少,分子量小,分布集中;在8个鉴定的蛋白点中,1个是30kP蛋白酶A原,5个是类胰蛋白酶,其它2个是碱性丝氨酸蛋白酶。
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| 关 键 词： | 家蚕 消化液 蛋白质组学 双向电泳 基质辅助激光解析电离飞行时间质谱 |
Proteomic Analysis of Digestive Juice From Silkworm (Bombyx mori)Larvae |
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| CUI Ying-Jun,QIU Zhi-Yong,ZHAO Qiao-Ling,SHEN Xing-Jia,TANG Shun-Ming,GUO Xi-Jie.Proteomic Analysis of Digestive Juice From Silkworm (Bombyx mori)Larvae[J].Acta Sericologica Sinica,2008,34(1):50-53. |
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| Authors: | CUI Ying-Jun QIU Zhi-Yong ZHAO Qiao-Ling SHEN Xing-Jia TANG Shun-Ming GUO Xi-Jie |
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| Abstract: | To investigate the constitutions of proteins of the digestive juice from silkworm(Bombyx mori) larvae,we separated the proteins by two dimensional electrophoresis(2-DE),identified 8 main proteins by means of matrix assisted laser desorption ionizationtiome of flight mass spectrometry(MALDI-TOF MS),and analyzed them through bioinformatics methods.The results showed that there are less proteins spots in the digestive juice,characterized as small molecular weight and similar pI.Among the identified 8 protein spots,1 protein spot is 30kP protease A,5 protein spots are trypsin-like protease,the other 2 protein spots are alkaliphilic serine protease P-IIc. |
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| Keywords: | Bombyx mori Digestive juice Proteomics 2-DE MALDI-TOF MS |
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