| 应用聚合酶链反应检测鸡传染性贫血病毒 |
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| 引用本文： | 谢芝勋,Khan,MI.应用聚合酶链反应检测鸡传染性贫血病毒[J].中国动物检疫,1999,16(6):1-3. |
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| 作者姓名： | 谢芝勋 Khan MI |
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| 作者单位： | 广西兽医研究所!南宁，530001，美因康涅狄格州大学兽医系 |
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| 摘 要： | 根据鸡传染性贫血病毒（CAV）Cux－1株的基因序列，设计了一对引物，用这对引物对三株CAV的核酸模板进行PCR扩增，结果均能特异性地扩增出420bp的片段，但对其它5种禽病病原体核酸模板的扩增，结果均为阴性，该PCR能检出10fg鸡传染性贫血病毒DNA模板。
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| 关 键 词： | 聚合酶链反应 鸡传染性贫血病毒 引物 |
Detection of Chicken Anemia Virus by Polymerase Chain Reaction |
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| Xie Zhixun, Mazhar I. Khan.Detection of Chicken Anemia Virus by Polymerase Chain Reaction[J].China Journal Of Animal Quarantine,1999,16(6):1-3. |
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| Authors: | Xie Zhixun Mazhar I Khan |
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| Abstract: | A polymerase chain reaction (PCR)for avian anemia virus was developed. The primers was designed and synthesized after the DNA sequence data of the Cux- 1 strain of chicken anemia virus. And chicken anemia virus specific 420 base pair DNA product was amplified by these primers, but not from by those other 5 avian pathogenic virus and bacteria. As low as 10 femtogram of avian anemia virus DNA was detected by this PCR. |
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| Keywords: | Avian Anemia Virus PCR DNA Primer |
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