| 糯玉米的成熟胚培养 |
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| 引用本文： | 何婷,王亦菲,陆瑞菊,等. 糯玉米的成熟胚培养[J]. 中国农学通报, 2009, 25(4): 47-49 |
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| 作者姓名： | 何婷 王亦菲 陆瑞菊 等 |
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| 作者单位： | 1. 上海市农业科学研究院生物技术研究所,上海,201106;上海市农业遗传育种重点实验室,上海,201106
2. 上海市农业科学研究院作物育种栽培研究所,上海,201106 |
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| 基金项目： | 上海市科学技术委员会重大项目 |
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| 摘 要： | 以糯玉米成熟胚为外植体作离体培养，研究在培养基中添加植物激素，硝酸银及有机物等对玉米愈伤组织的诱导和胚性愈伤继代生长的影响。结果表明：在诱导培养阶段，N6+6-BA 0.2mg/L+2,4-D 2mg/L的培养基诱导率最高，愈伤质量好；在继代培养阶段，MS+6-BA 0.2mg/L+2,4-D 2mg/L +AgNO310mg/L或CW (Coconut Water) 10%的培养基可改善胚性愈伤的质量。
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| 关 键 词： | 土壤 土壤 肥料 配方施肥 信息技术 |
| 收稿时间： | 2008-12-01 |
| 修稿时间： | 2008-12-30 |
Mature Embryo Culture in Waxy Maize |
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| He Ting,Wang Yi-fei,Lu Rui-ju,Chen Zhi-wei,Du Zhi-zhao,Zhang Yan-min,Huang Jian-hua,Shen Xue-fang,Wang Yi-fa. Mature Embryo Culture in Waxy Maize[J]. Chinese Agricultural Science Bulletin, 2009, 25(4): 47-49 |
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| Authors: | He Ting Wang Yi-fei Lu Rui-ju Chen Zhi-wei Du Zhi-zhao Zhang Yan-min Huang Jian-hua Shen Xue-fang Wang Yi-fa |
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| Affiliation: | He Ting ;Wang Yifei ;Lu Ruiju ;Chen Zhiwei ;Du Zhizhao ;Z hang Yanmin ;Huang Jianhua ;Shen Xuefang ;Wang Yifa (1 Biotech Research Institute;Shanghai Academy of Agricultural Sciences;Shanghai 201106;2 Shanghai Key Laboratory of Agricultural Genetics and Breeding;Shanghai 201106;3 Crop Breeding and Cultivation Research Institute;Shanghai Academy of Agricultural Sciences;Shanghai 201106) |
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| Abstract: | Mature waxy maize embryos were cultured as explants in vitro, and the effects on callus induction and subculturing were investigated under culture mediums with different additives, such as plant hormones, AgNO3, organic compounds, etc. The results showed that the combination of N6 + 0.2mg/L 6-BA + 2mg/L 2,4-D could obtain the most calli of higher quality in the induction phase, while the combination of MS + 0.2mg/L 6-BA + 2mg/L 2,4-D +10mg/L AgNO3 or 10% CW could make sure the calli of higher quality further in the stage of subculturing. Key words: Waxy maize, Mature embryo, Embryogenic callus |
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| Keywords: | waxy maize mature embryo embryogenic callus |
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