| 组织型纤溶酶原激活剂cDNA真核表达质粒的构建与鉴定 |
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| 引用本文： | 李银聚 扈荣良. 组织型纤溶酶原激活剂cDNA真核表达质粒的构建与鉴定[J]. 河南农业大学学报, 1998, 32(4): 335-338,344 |
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| 作者姓名： | 李银聚 扈荣良 |
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| 作者单位： | [1]洛阳农业高等专科学校 [2]解放军农牧大学 |
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| 摘 要： | 将组织型纤溶酶原激活剂基因ｃＤＮＡ经多次亚克隆插入到真核表达载体ｐＳＶＬ的ＳＶ４０启动子和ｐｃＤＮＡ３的ＣＭＶ启动子下游，构建了重组表达质粒ｐＳＶＬ－ｔＰＡ和ｐｃＤＮＡ３－ｔＰＡ。经酶切和Ｓｏｕｔｈｅｒｎ杂交鉴定，ｔ－ＰＡ基因的插向正确，可用于哺乳动物细胞和个体表达系统中表达。
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| 关 键 词： | 组织型 纤溶酶原激活剂 cDNA 真核表达 质粒构建 |
Construction and Identification of t-PA cDNA Gene Expressing Plasmids |
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| Li Yinju Deng Wen Pang Youzhi et al. Construction and Identification of t-PA cDNA Gene Expressing Plasmids[J]. Journal of Henan Agricultural University, 1998, 32(4): 335-338,344 |
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| Authors: | Li Yinju Deng Wen Pang Youzhi et al |
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| Abstract: | Two expressing plasmids,pSVL-tPA and pcDNA3-tPA,containing the tissue-type plasminogen activator (t-PA) cDNA gene,were constructed by inserting the t-PA cDNA into the downstream of the SV40 promoter and the CMV promoter,respectively.Restriction enzymes digestion and southern bolt demonstrated that t-PA gene had been inserted into right site.The recombinant plasmids could be used as expressing vectors in mammalian cells. |
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| Keywords: | t-PA cDNA expressing plasmid southern blot |
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