猪乳腺细胞分离培养及EGFP基因转化

本研究旨在从猪乳腺组织中分离得到上皮细胞和成纤维细胞,并将EGFP基因导入这些细胞.利用乳腺细胞堵养体系从成年猪乳腺组织中分离培养上皮细胞和成纤维细胞,并利用脂质体介导转染技术将EGFP基因导入这些细胞.结果,从成年猪乳腺组织中成功分离培养出上皮细胞和成纤维细胞,获得转EGFP基因上皮细胞和成纤维细胞.上皮细胞呈短梭形或多角形,细胞之间紧密相靠,互相衔接,连接成片;细胞核呈圆形或椭圆形,核仁2～4枚,比较明显.成纤维细胞呈长梭形.结果表明,可以从猪乳腺组织中分离上皮细胞和成纤维细胞,EGFP可以在这些细胞中表达.

Isolation and EGFP Gene Transfection of Porcine Mammary Gland Cells

This experiment was conducted to isolate porcine mammary gland epithelial and fibroblast cells from adult porcine mammary gland tissue, and transfect the enhanced green fluorescent protein (EGFP) gene into the cells. Porcine mammary gland epithelial and fibroblast cells were isolated from adult porcine mammary gland tissue using their culture system. EGFP gene was transfected into the cells by lipofection technology. We succeeded in isolating porcine mammary gland epithelial and fibroblast cells from adult porcine mammary gland tissue, and the EGFP gene transfected epithelial and fibroblast cells were obtained. The epithelial cells were short shuttie-like or guboidal, which were connected with each other closely and properly. The cell nucleus were circular or oval which had 2-4 cores. The fibroblast cells were long shuttle-like. This result shows that porcine epithelial and fibroblast cells could be isolated from adult porcine mammary gland tissue and EGFP could be expressed in the cells.