| 花叶病毒侵染的果蔗(Badila)的基因表达 |
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| 引用本文： | 周以飞,戴艳,潘大仁,陈观水,高毅. 花叶病毒侵染的果蔗(Badila)的基因表达[J]. 福建农林大学学报(自然科学版), 2006, 35(3): 288-291 |
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| 作者姓名： | 周以飞 戴艳 潘大仁 陈观水 高毅 |
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| 作者单位： | 福建农林大学作物科学学院,福建,福州,350002 |
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| 基金项目： | 中国科学院资助项目;福建省自然科学基金 |
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| 摘 要： | 采用荧光标记差异显示技术(d ifferential d isp lay,简称DD-PCR)对甘蔗花叶病毒(Sugarcane m osaic virus,SCMV)侵染的果蔗(Bad ila)基因表达进行研究.用随机筛选出的23对引物组合扩增出9条差异条带,其中4条是有明显差异表达的cDNA条带,5条是表达强弱的差异条带.同时对一些影响荧光标记DD-PCR的因素进行了分析.
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| 关 键 词： | 果蔗 甘蔗花叶病毒 差异显示(DD) 基因表达 |
| 文章编号： | 1671-5470(2006)03-0288-04 |
| 收稿时间： | 2006-03-11 |
| 修稿时间： | 2006-04-05 |
Gene expression in Badila infected by SCMV by means of differential display |
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| ZHOU Yi-fei,DAI Yan,PAN Da-ren,CHEN Guan-shui,GAO Yi. Gene expression in Badila infected by SCMV by means of differential display[J]. Journal of Fujian Agricultural and Forestry University, 2006, 35(3): 288-291 |
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| Authors: | ZHOU Yi-fei DAI Yan PAN Da-ren CHEN Guan-shui GAO Yi |
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| Affiliation: | College of Crop Science, Fujian Agrieuhure and Forestry University, Fuzhou, Fujian 350002, China |
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| Abstract: | Nine differentially expressed cDNA fragments were obtained with 23 primer combinations,the sizes of their fragments were from 250 to 350 bp.These fragments were divided into two types: 4 cDNA fragments could differentially expressed in quality;5 cDNA fragments could differentially expressed in intensity.Some factors affecting the technology were also analyzed. |
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| Keywords: | Badila Sugarcane mosaic virus(SCMV) differential display(DD) gene expression |
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