镉对大鼠原代培养肝细胞毒性的研究 |
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引用本文: | 杨建泉,马宏兵,刘学忠.镉对大鼠原代培养肝细胞毒性的研究[J].现代农业科技,2012(15):266-267,269. |
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作者姓名: | 杨建泉 马宏兵 刘学忠 |
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作者单位: | 1. 江苏省如东县畜牧兽医站,江苏如东,226400 2. 如东县洋口畜牧兽医站 3. 扬州大学兽医学院 |
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摘 要: | 为探讨镉对原代大鼠肝细胞的毒性作用。用二步灌流法获得大鼠肝细胞,肝细胞暴露于浓度为2.5、5.0、10.0、20.0μmoL/L的醋酸镉中12、24 h,应用四甲基偶氮噻唑蓝(MTT)比色法检测醋酸镉对肝细胞存活率的影响及培养上清液中乳酸脱氢酶(LDH)的活性变化。结果表明,肝细胞暴露于浓度为2.5、5.0、10.0、20.0μmoL/L的醋酸镉中,细胞相对存活率显著下降(P0.01),LDH的释放量增加,且具有剂量效应关系。提示一定浓度的醋酸镉可引起肝细胞的毒性损伤。
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关 键 词: | 大鼠 肝细胞 醋酸镉 毒性 |
Study on Toxicity of Cadmium on Primary Culture Hepatocytes in Rats |
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Authors: | YANG Jian-quan MA Hong-bing LIU Xue-zhong |
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Institution: | 1 Animal Husbandry and Veterinary Station of Rudong County in Jiangsu Province,Rudong Jiangsu 226400;2 Yangkou Animal Husbandry and Veterinary Station of Rudong County;3 College of Veterinary Medicine,Yangzhou University) |
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Abstract: | In order to study the toxical effects of cadmium on primary culture hepatocytes in rats.Rat hepatocytes were isolated by a two step perfusion technique,the hepatocytes were incubated with cadmium acetate(2.5 μmoL/L,5.0 μmoL/L,10.0 μmoL/L and 20.0 μmoL/L)for 12 h and 24 h,methylthiazolyldiphenyl-tetrazolium bromide(MTT)technique was used to determine hepatocytes cell viability in this study.The content of lactate dehydrogenase(LDH)in hepatocyte primary culture fluid were determined.The results showed that cell viability decreased significantly(P<0.01)in a dose dependent manner after exposure to 2.5 μmoL/L,5.0 μmoL/L,10.0 μmoL/L and 20.0 μmoL/L cadmium acetate,the activities of LDH increased.It suggested that cadmium acetate may induce the damage of hepatocytes at certain doses. |
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Keywords: | hepatocytes cadmium acetate toxicity |
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