| 水稻干燥组织DNA模板的快速制备 |
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| 引用本文： | 田永航,罗洪发.水稻干燥组织DNA模板的快速制备[J].中国农学通报,2010,26(11):32-35. |
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| 作者姓名： | 田永航 罗洪发 |
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| 作者单位： | 贵州大学农学院,贵阳,550025 |
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| 摘 要： | 用碱处理水稻干燥组织，浸出液直接用作PCR扩增的模板，扩增结果稳定、准确，与CTAB法和SDS法提取的DNA扩增效果相比没有显著差异。用此法提取水稻自然干燥的叶片、叶鞘、茎杆、颖壳和根DNA，质量良好，能够满足SSR分析要求。
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| 关 键 词： | 氮肥用量 氮肥用量 栽插密度 水稻 茎秆力学特性 影响 |
| 收稿时间： | 2010-01-13 |
| 修稿时间： | 2010-02-19 |
The Rapid Extraction of Rice Genomic DNA from Natural Dried Tissue |
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| Tian Yonghang,Luo Hongfa.The Rapid Extraction of Rice Genomic DNA from Natural Dried Tissue[J].Chinese Agricultural Science Bulletin,2010,26(11):32-35. |
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| Authors: | Tian Yonghang Luo Hongfa |
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| Institution: | Agricultural college, Guizhou University, Guiyang 550025 |
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| Abstract: | After the alkali treatment for rice natural dried tissues, taking the alkaline leaching solution directly as a template for PCR amplification, the amplification results are stable, accurate, and are insignificantly different in comparison with the methods CTAB and SDS. The DNA extracted from natural dried rice flag leaf, leaf sheath ,culm ,glume husk and roots ,have good quality, and can meet with the SSR analysis. |
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| Keywords: | rice PCR DNA template methodzz |
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