| 铁皮石斛组培快繁技术的研究 |
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| 引用本文： | 曹天旭,宿肇辉,韩超慧. 铁皮石斛组培快繁技术的研究[J]. 黑龙江农业科学, 2014, 0(5): 30-34 |
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| 作者姓名： | 曹天旭 宿肇辉 韩超慧 |
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| 作者单位： | [1]黑龙江农业经济职业学院,黑龙江牡丹江157041 [2]牡丹江市第二人民医院,黑龙江牡丹江157000 |
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| 摘 要： | 为了建立铁皮石斛快速繁殖体系,以其茎段为材料,研究了不同培养基组分、激素浓度对铁皮石斛增殖和生根的影响。结果表明:铁皮石斛在初代培养中用酒精消毒30s后再用0.1%升汞杀菌6min最为适宜;最适培养基为MS基本培养基;在MS+1.0mg·L-1 6-BA+0.5mg·L-1 NAA中铁皮石斛的增殖效果最佳,在MS培养基中添加3.0mg·L-1 IBA生根效果最好,生根率可达100%;试管苗移栽的最佳基质为苔藓,单独使用成活率可达90.6%。
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| 关 键 词： | 铁皮石斛 丛生芽 组织培养 |
Study on Tissue Culture and Rapid Propagation of Dendrobium candidum |
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| Affiliation: | CAO Tian-xu1 , SU Zhao-hui2 , ItAN Chao-hui (1. Heilongjiang Agricultural Economy Vocational College, Mudanjiang, Heilongjiang 157041 ; 2. Mudanjiang Second People＇s Hospital, Mudanjiang, Heilongjiang 157000) |
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| Abstract: | In order to establish the rapid propagation system of Dendrobium candidum, taking its stem as materials,the effects of medium types,hormone concentration on proliferation and rooting of Dendrobium candidum were studied. The results showed that the best sterilization method for explants was to treat by 75 % ethyl alcohol for 30 s and then 0. 1% HgCl2 for 6 min. The optimum basic medium was MS. The greatest number of propagation and shoot forming were found in the MS medium supplemented with 1 mg·L-1 6-BA and 0.5 mg·L-1 NAA. Roots were easily formed by the addition of IBA,and the treatment of 3.0 mg·L-1 IBA in MS medium was more effective for growth of roots in vitro. The planting survival rate achieved 90.6 ; in simplex growing medium with moss,it was higher than the other growing media. |
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| Keywords: | Dendrobium candidum cluster-buds tissue culture |
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