Diagnosis of paratuberculosis in goats by cell mediated immune response,conventional and molecular diagnostic techniques |
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| Authors: | Rawther Sulficar Shamsudeen Saseendranath Mapranath Raghavan Nair Govinda Pilla Krishnan Tresamol Palathingal Vareid Pillai Usha Narayana Abraham John Senthilkumar Tuticorin Maragatham Alagesan Nagalakshmy Shrine Nimisha Krishna Kripa Narayanan |
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| Institution: | (1) Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy, Trichur, Kerala, India;(2) Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Trichur, Kerala, India;(3) Department of Veterinary Clinical Medicine, Jurisprudence and Ethics, College of Veterinary and Animal Sciences, Mannuthy, Kerala Agricultural University, Trichur, Kerala, India;(4) Department of Livestock Production Management, College of Veterinary and Animal Sciences, Pookot, Wayanad, Kerala Agricultural University, Trichur, Kerala, India;(5) Department of Animal Biotechnology, Madras Veterinary College, Chennai, Tamil Nadu, India;(6) Department of Veterinary Epidemiology and Preventive Medicine, Veterinary College and Research Institute, Namakkal, Tamil Nadu, India |
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| Abstract: | In the present study efficacy of single intradermal Johnin test, acid fast staining of faecal smear and IS 900 faecal polymerase chain reaction tests was evaluated in 200 goats for detection of Mycobacterium avium subsp paratuberculosis. Two hundred goats comprising 150 goats from an organised farm in Trichur district and 50 goats reared under field condition
at farmers premise from Malappuram district of Kerala state formed the study population. Faecal smear from all the 200 goats
was stained by Ziehl–Neelsen acid fast stain and faecal polymerase chain reaction (PCR) specific for M. avium subsp paratuberculosis (MAP); IS 900 was performed on all samples. All the animals were subjected to single intradermal Johnin test. Out of 200 goats screened
for paratuberculosis, six goats (3%), 11 goats (5.5%) and 42 goats (21%) were found positive by Ziehl–Neelsen acid fast staining
of faecal smear, single intradermal Johnin test and IS 900 PCR respectively. Results of the present study indicate that amplification of IS 900 insertion element was the most specific and sensitive diagnostic detection method. Single intradermal Johnin test and Ziehl–Neelsen
acid fast staining did not show any significant difference. |
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