| 苋菜愈伤组织诱导的研究 |
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| 引用本文： | 刘生财,杨琼,赖钟雄.苋菜愈伤组织诱导的研究[J].河南农业科学,2013,42(1):90-93. |
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| 作者姓名： | 刘生财 杨琼 赖钟雄 |
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| 作者单位： | 福建农林大学园艺植物生物T程研究所,福建福州,350002 |
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| 基金项目： | 国家科技支撑计划项目(2007BAD07B03);福建农林大学园艺学博士后基金项目;福建农林大学校青年教师基金项目(2011xjj04) |
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| 摘 要： | 以苋菜种子为试材诱导无菌苗,取幼苗期下胚轴、子叶和壮苗期茎段、叶片作为外植体,比较不同组织部位对愈伤组织诱导效果的影响,并探讨不同质量浓度NAA对苋菜愈伤组织诱导的影响。结果表明,以苋菜壮苗期叶片和茎段为外植体的愈伤组织诱导率比幼苗期下胚轴和子叶高,表现为叶片>茎段>下胚轴>子叶;苋菜壮苗期茎段、叶片在MS+6-BA 0.5mg/L+NAA 3.0mg/L培养基上诱导愈伤组织的效果最好,诱导率分别为98.7%和99.3%。
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| 关 键 词： | 苋菜 愈伤组织 诱导 苗期 |
Study on Callus Induction from Amaranthus tricoclor L. |
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| LIU Sheng-cai
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YANG Qiong
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LAI Zhong-xiong.Study on Callus Induction from Amaranthus tricoclor L.[J].Journal of Henan Agricultural Sciences,2013,42(1):90-93. |
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| Authors: | LIU Sheng-cai YANG Qiong LAI Zhong-xiong |
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| Institution: | (Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China) |
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| Abstract: | In this experiment,seeds of Amaranthus tricoclor L.were used to produce plantlets.The differences in callus induction were compared among hypocotyls,cotyledons of young seedling stage and stem segments,leaves of adult seedling stage.Meanwhile,the effects of the different concentrations of NAA on callus induction were analyzed.The results showed that calli occurred better at the adult seedling stage than at young seedling stage.The order of induction effects as explants was:leaves>stem segments>hypocotyls>cotyledons.The best medium for callus induction from stem segments and leaves of the adult seedling stage was MS medium supplemented with 0.5 mg/L 6-BA and 3.0 mg/L NAA,on which the induction rate was 98.7% and 99.3%,respectively. |
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| Keywords: | Amaranthus tricoclor L callus induction seedling period |
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