| 斑马鱼发育过程中DANA相关基因的定量分析 |
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| 引用本文： | 韩亚伟,汤永涛,陈利平,左婷婷.斑马鱼发育过程中DANA相关基因的定量分析[J].河南农业科学,2013,42(1):132-134. |
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| 作者姓名： | 韩亚伟 汤永涛 陈利平 左婷婷 |
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| 作者单位： | 郑州轻工业学院食品与生物工程学院,河南郑州,450002 |
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| 基金项目： | 国家自然科学基金项目(31112175) |
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| 摘 要： | DANA是广泛分布于斑马鱼基因组中的一个短散在核重复序列家族,通过实时荧光定量PCR相对定量方法,检测与DANA相关基因mul1a、er-Gicp2在斑马鱼整个个体发育过程中的相对表达量。结果显示:mul1a基因在1细胞期到1 000细胞期,即早期卵裂期内表达量差异不明显,从sphere时期到成体时期,其表达量显著性下调,并在shield时期降到最小值,为1细胞时期的0.009倍;er-Gicp2基因从2细胞到成体时期持续下调,到成体时期其表达量只有1细胞时期的0.023倍。
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| 关 键 词： | 斑马鱼 个体发育 荧光定量PCR |
Quantitative Analysis of Expressions of DANA Related Genes during Development of Zebrafish |
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| HAN Ya-wei
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TANG Yong-tao
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CHEN Li-ping
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ZUO Ting-ting.Quantitative Analysis of Expressions of DANA Related Genes during Development of Zebrafish[J].Journal of Henan Agricultural Sciences,2013,42(1):132-134. |
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| Authors: | HAN Ya-wei TANG Yong-tao CHEN Li-ping ZUO Ting-ting |
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| Institution: | (School of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450002,China) |
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| Abstract: | DANA is a family of short interspersed nuclear elements,which widely exists in zebrafish genome.The relative expressions of DANA related genes,mul1a and er-Gicp2,were detected in zebrafish ontogenesis by real-time fluorescence quantitative PCR.The results showed that the relative expression of mul1a had no obvious difference from 1 cell stage to 1 000 cell stage,but obviously down-regulated from sphere stage and reached the lowest at shield stage,only about 0.009 times as that of 1 cell stage.The relative expression of er-Gicp2 gene is persistently down-regulated from 1 cell stage to adult,and the relative expression of adult was only 0.023 times as that of 1 cell stage. |
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| Keywords: | zebrafish ontogenesis real-time fluorescence quantitative PCR |
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