Detection of antibodies to Eperythrozoon ovis by the use of an enzyme-linked immunosorbent assay |
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| Authors: | F M Lang G R Ferrier T J Nicholls |
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| Affiliation: | Victorian Department of Agriculture and Rural Affairs, Regional Veterinary Laboratory, Benalla, Australia. |
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| Abstract: | Specific antibody to Eperythrozoon ovis was detected by an enzyme-linked immunosorbent assay (ELISA) in the sera of infected sheep. In the presence of parasite antigen, positive control serum showed a reaction approximately eight times that of negative serum. When compared to an immunofluorescent antibody test (IFAT), the ELISA was eight times more sensitive. Positive control sera gave a titre of 1:3200 by IFAT and 1:25,600 by ELISA. Through the use of a reference titration curve ELISA could be used as a semi-quantitative system to determine antibody levels in test sera. |
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