| 中原黄牛GHR基因5'端PCR-RFLP多态性分析 |
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| 引用本文： | 张黎鑫,殷宗俊,陈宏权,王力生. 中原黄牛GHR基因5'端PCR-RFLP多态性分析[J]. 中国奶牛, 2010, 0(8): 1-3 |
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| 作者姓名： | 张黎鑫 殷宗俊 陈宏权 王力生 |
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| 作者单位： | 安徽农业大学动物科学系,合肥,230036 |
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| 基金项目： | 安徽省"十一五"科技攻关项目，国家科技支撑计划项目 |
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| 摘 要： | 本文采用两种限制性内切酶(Cfr13 I和Sat I)对我国中原黄牛生长激素受体(GHR)基因5’端部分序列进行PCR-RFLP分析。结果表明:在Cfr13 I酶切位点上,GHR基因有CC、CT、TT三个基因型,C等位基因频率为0.829,T等位基因为0.171,χ2检验表明在此位点符合Hardy-Weinberg平衡,多态信息含量为0.236;在Sat I酶切位点上,GHR基因有CC、CT、TT三个基因型,C等位基因频率为0.849,T等位基因为0.151,χ2检验表明在此位点符合Hardy-Weinberg平衡,多态信息含量小、为0.229。
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| 关 键 词： | 中原黄牛 GHR基因 PCR-RFLP |
Polymorphism of 5'-Region in the GHR Gene of Chinese Yellow Cattle with PCR-RFLP |
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| ZHANG Li-xin,YIN Zong-jun,CHEN Hong-quan,WANG Li-sheng. Polymorphism of 5'-Region in the GHR Gene of Chinese Yellow Cattle with PCR-RFLP[J]. China Dairy Cattle, 2010, 0(8): 1-3 |
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| Authors: | ZHANG Li-xin YIN Zong-jun CHEN Hong-quan WANG Li-sheng |
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| Affiliation: | (Department of Animal Science, Anhui Agricultural University, Hefei 230036) |
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| Abstract: | In this experiment, 5＇-region in the GHR gene of Chinese yellow cattle was studied by PCR-RFLP with two restriction enzyme Cfrl3 I and Sat I. The results showed that： three genotype CC, CT, TT were found in Cfrl3 I polymorphic site, the gene frequency of C and T were 0.829 and 0.171 respectively. The result of X2 test indicated that it was accorded with Hardy-Weinberg equilibrium, and polymorphism information content was 0.236. Three genotype CC,CT,TT were found in Sat I polymorphic site, the gene frequency of C and T were 0.849 and 0.151 respectively. The result of X2 test indicated that it was accorded with Hardy-Weinberg equilibrium, and polymorphism information content was 0.229. |
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| Keywords: | PCR-RFLP |
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