| 水貂株犬瘟热病毒融合蛋白基因的克隆与序列分析 |
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| 引用本文： | 苏凤艳,宗春苗,王卓聪,丁庆东,王全凯. 水貂株犬瘟热病毒融合蛋白基因的克隆与序列分析[J]. 西北农业学报, 2008, 17(6): 21-24 |
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| 作者姓名： | 苏凤艳 宗春苗 王卓聪 丁庆东 王全凯 |
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| 作者单位： | 吉林农业大学,中药材学院,吉林长春,130118 |
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| 摘 要： | 以犬瘟热病毒水貂分离株RNA为模板,经RT-PCR反应,扩增出融合蛋白基因的1053bp DNA片段,通过T-A克隆技术,成功构建克隆载体PMD-18T/F。序列分析表明:分离株与01-2689株、2544-Han95株、A75-17株、DOGDK91C株、00-2601株、ONP株、PDV-2株核苷酸序列同源性分别为94.3%、93.5%、94.4%、93.6%9、5.3%、97.3%和94.5%;氨基酸序列同源性分别为97.2%、96.6%、97.4%、97.4%、97.4%、97.2%和98.0%。抗原指数分析表明分离株与疫苗ONP株、强毒A75-17株在40-50位氨基酸间存在明显差异。
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| 关 键 词： | 水貂 犬瘟热病毒 融合蛋白基因 克隆 序列分析 |
| 收稿时间： | 2008-01-14 |
| 修稿时间： | 2008-04-29 |
Cloning and Sequence Analysis of Fusion Protein Gene of Canine Distemper Virus Isolated from Mink |
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| SU Feng-yan,ZONG Chun-miao,WANG Zhuo-cong,DING Qing-dong and WANG Quan-kai. Cloning and Sequence Analysis of Fusion Protein Gene of Canine Distemper Virus Isolated from Mink[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2008, 17(6): 21-24 |
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| Authors: | SU Feng-yan ZONG Chun-miao WANG Zhuo-cong DING Qing-dong WANG Quan-kai |
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| Affiliation: | Chinese Medicinal Materials College, Jilin Agricultural University, Changchun 130118, China;Chinese Medicinal Materials College, Jilin Agricultural University, Changchun 130118, China;Chinese Medicinal Materials College, Jilin Agricultural University, Changchun 130118, China;Chinese Medicinal Materials College, Jilin Agricultural University, Changchun 130118, China;Chinese Medicinal Materials College, Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | |
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| Keywords: | Mink Canine distemper virus Fusion protein gene Cloning Sequence analysis |
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