Immunological study of an attenuated Salmonella Typhimurium
expressing ApxIA,ApxIIA, ApxIIIA and OmpA of Actinobacillus
pleuropneumoniae in a mouse model |
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Authors: | Jin HUR Seong Kug EO Sang-Youel PARK Yoonyoung CHOI John Hwa LEE |
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Affiliation: | 1)Department of Bioactive Material Sciences, and Department of Veterinary Public Health, College of Veterinary Medicine, Chonbuk National University, Jeonju 561–756, South Korea |
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Abstract: | Salmonella Typhimurium strain expressing the Actinobacilluspleuropneumoniae antigens, ApxIA, ApxIIA, ApxIIIA and OmpA, was previouslyconstructed as a vaccine candidate for porcine pleuropneumonia. This strain was a liveattenuated (∆lon∆cpxR∆asd)Salmonella as a delivery hostand contained a vector containing asd. An immunological study oflymphocyte proliferation, T-lymphocyte subsets and cytokines in the splenocytes of a mousemodel was carried out after stimulation with the candidate SalmonellaTyphimurium by intranasal inoculation. The splenic lymphocyte proliferation and the levelsof IL-4, IL-6 and IL-12 of the inoculated mice were significantly increased, and the T-and B-cell populations were also elevated. Collectively, the candidate may efficientlyinduce the Th1- and Th2-type immune responses. |
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Keywords: | Actinobacillus pleuropneumoniae porcine pleuropneumonia Salmonella Typhimurium mutant |
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