| 嗜水气单胞菌拮抗菌甲基营养型芽孢杆菌WM-1发酵条件优化 |
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| 引用本文： | 张皎皎,马富平,李艳红,吴正理,姚维志.嗜水气单胞菌拮抗菌甲基营养型芽孢杆菌WM-1发酵条件优化[J].水产学报,2018,42(7):1124-1131. |
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| 作者姓名： | 张皎皎 马富平 李艳红 吴正理 姚维志 |
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| 作者单位： | 西南大学动物科技学院;西南大学渔业资源环境研究中心;淡水鱼类资源与生殖发育教育部重点实验室西南大学 |
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| 基金项目： | 国家自然科学基金（31770965，31600716）；中央高校基本科研业务费专项（XDJK2015A001，SWU114011，SWU114015） |
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| 摘 要： | 为优化嗜水气单胞菌拮抗菌——甲基营养型芽孢杆菌WM-1的发酵条件,获得抑菌物质产生的最佳培养基配方和培养条件,本实验以嗜水气单胞菌为指示菌,以发酵上清液的抑菌活性为检测指标,采用单因素和正交实验设计,对菌株WM-1的发酵条件进行优化。结果显示,菌株WM-1最适发酵培养基为可溶性淀粉1.0%、牛肉膏1.5%、硫酸镁0.07%、磷酸氢二钾0.05%;最佳发酵条件为初始pH 7.5、培养温度30°C、装液量20%、摇床转速210 r/min、培养时间48 h。在最适培养基和最佳发酵条件下,菌株WM-1发酵液抑菌圈直径达(22.5±0.50)mm,抑菌活性比优化前提高了40.6%。本研究为嗜水气单胞菌的防治提供了新的材料、为甲基营养型芽孢杆菌应用于嗜水气单胞菌的生物防控提供了理论基础。
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| 关 键 词： | 甲基营养型芽孢杆菌 嗜水气单胞菌 拮抗菌 发酵条件 优化 |
| 收稿时间： | 2016/12/26 0:00:00 |
| 修稿时间： | 2017/5/4 0:00:00 |
Fermentation optimization of a strain Bacillus methylotrophilus WM-1 against Aeromonas hydrophila |
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| ZHANG Jiaojiao,MA Fuping,LI Yanhong,WU Zhengli and YAO Weizhi.Fermentation optimization of a strain Bacillus methylotrophilus WM-1 against Aeromonas hydrophila[J].Journal of Fisheries of China,2018,42(7):1124-1131. |
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| Authors: | ZHANG Jiaojiao MA Fuping LI Yanhong WU Zhengli and YAO Weizhi |
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| Institution: | College of Animal Science and Technology, Southwest University, Chongqing 400715, China;Research Center of Fishery Resources and Environment, Southwest University, Chongqing 400715, China,College of Animal Science and Technology, Southwest University, Chongqing 400715, China;Research Center of Fishery Resources and Environment, Southwest University, Chongqing 400715, China,College of Animal Science and Technology, Southwest University, Chongqing 400715, China;Research Center of Fishery Resources and Environment, Southwest University, Chongqing 400715, China,College of Animal Science and Technology, Southwest University, Chongqing 400715, China;Research Center of Fishery Resources and Environment, Southwest University, Chongqing 400715, China;Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Southwest University, Chongqing 400715, China and College of Animal Science and Technology, Southwest University, Chongqing 400715, China;Research Center of Fishery Resources and Environment, Southwest University, Chongqing 400715, China;Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Southwest University, Chongqing 400715, China |
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| Abstract: | |
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| Keywords: | Bacillus methylotrophilus Aeromonas hydrophila antagonistic bacteria fermentation condition optimization |
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