| 急性氨氮暴露对大弹涂鱼炎性反应相关基因表达的影响 |
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| 引用本文： | 宋美泽,黎明,李健,袁莉霞,王日昕,石戈.急性氨氮暴露对大弹涂鱼炎性反应相关基因表达的影响[J].水产学报,2018,42(11):1704-1710. |
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| 作者姓名： | 宋美泽 黎明 李健 袁莉霞 王日昕 石戈 |
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| 作者单位： | 浙江海洋大学海洋科学与技术学院;宁波大学海洋学院 |
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| 基金项目： | 国家自然科学基金（31472279；31502176）；宁波市自然科学基金（2016A610083） |
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| 摘 要： | 为研究急性氨氮胁迫对大弹涂鱼炎性反应相关基因表达的影响,实验挑选初始体质量为(15.14±0.05) g的健康大弹涂鱼幼鱼180尾,进行96 h的急性氨氮胁迫实验。结果显示,大弹涂鱼96 h氨氮半致死浓度为8.99 mg/L总氨氮(0.11 mg/L非离子氨,T-AN);氨氮胁迫后TNF基因的mRNA表达量分别于12和96 h时显著上调,96 h时表达量达到0 h时的2倍;IL-1基因的mRNA表达量12 h时显著上调,为0 h时表达量的6倍;氨氮胁迫后IL-6基因的mRNA表达量分别于12和96 h时显著上调,表达量达到0 h时的1.5倍;氨氮胁迫后IL-8基因的mRNA表达量在24 h时出现显著下调。研究表明,大弹涂鱼96 h氨氮半致死浓度为8.99 mg/L总氨氮;半致死浓度的氨氮胁迫48 h后,TNF、IL-1、IL-6和IL-8基因的mRNA表达量持续升高,推测过度炎性应激可能是导致鱼类氨中毒死亡的原因之一。
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| 关 键 词： | 大弹涂鱼 氨氮 基因 促炎性因子 |
| 收稿时间： | 2017/6/5 0:00:00 |
| 修稿时间： | 2017/7/26 0:00:00 |
Effects of acute ammonia exposure on gene involved in inflammation of mudskipper |
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| SONG Meize,LI Ming,LI Jian,YUAN Lixi,WANG Rixin and SHI Ge.Effects of acute ammonia exposure on gene involved in inflammation of mudskipper[J].Journal of Fisheries of China,2018,42(11):1704-1710. |
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| Authors: | SONG Meize LI Ming LI Jian YUAN Lixi WANG Rixin and SHI Ge |
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| Institution: | Marine Sciences Technology College, Zhejiang Ocean University, Zhoushan 316000, China,School of Marine Sciences, Ningbo University, Ningbo 315211, China,Marine Sciences Technology College, Zhejiang Ocean University, Zhoushan 316000, China,School of Marine Sciences, Ningbo University, Ningbo 315211, China,School of Marine Sciences, Ningbo University, Ningbo 315211, China and Marine Sciences Technology College, Zhejiang Ocean University, Zhoushan 316000, China |
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| Abstract: | A study was carried out to test the effects of acute ammonia exposure on gene involved in inflammation of mudskipper Boleophthalmus pectinirostris. The mudskipper (15.14±0.05) g were randomly allocated to 2 groups (control group and exposure group) in triplicate for 96 h ammonia exposure. The 96 h lethal concentration 50 (LC50) of ammonia in mudskipper is 8.99 mg/L total ammonia nitrogen (TAN; 0.11 mg/L non-ionic ammonia); Fish exposed to ammonia showed the mRNA expression of TNF increased significantly by 2-fold for 96 h; The mRNA expression of IL-1 increased significantly by 6-fold for 12 h; And the mRNA expression of IL-6 increased significantly by 1.5 fold for 12 h and 96 h; however, the mRNA expression of IL-8 decreased significantly for 24 h. This study indicated that the 96 h LC50 of ammonia in mudskipper is 8.99 mg/L TAN; The mRNA expression of TNF, IL-1, IL-6, IL-8 increased significantly after fish exposed to ammonia for 48 h; The inflammation is considered as one of major causes of fish ammonia poisoning. |
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| Keywords: | Boleophthalmus pectinirostris ammonia gene inflammatory factor |
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