云南、西藏与新疆小麦醇溶蛋白Gli-1和Gli-2编码位点等位基因组成及遗传多样性分析

利用酸性聚丙烯酰胺凝胶电泳(A-PAGE)法,分析了64份中国西部特有小麦材料的醇溶蛋白编码位点等位基因组成及遗传多样性。结果表明,在34份云南小麦、24份西藏小麦和6份新疆小麦的G li-1位点上,分别发现了26、33和3个等位基因;在G li-2位点上,则分别发现15、16和3个等位基因。在云南和西藏小麦的G li-B 1、G li-D 1和G li-A 2位点上均发现了一些现有小麦醇溶蛋白编码基因位点目录中未列出的等位变异。从染色体组来看,云南小麦、西藏小麦和新疆小麦B染色体组的N ei s平均遗传变异系数高于A染色体组和D染色体组,这说明B染色体组的遗传变异要高于A和D染色体组。云南小麦、西藏小麦和新疆小麦群体内的N ei s平均遗传变异系数分别为0.6824、0.7471和0,说明云南小麦和西藏小麦群体具有较高的遗传多样性。

Analysis of Gliadin Allelic Composition at Gli-1 and Gli-2 Loci and Genetic Diversity in Yunnan, Tibetan and Xinjiang Wheats

Gliadin allelic composition and genetic diversity at Gli-1 and Gli-2 loci were investigated in 64 accessions of unique wheats from western China using acid polyacrylamide gel electrophoresis(A-PAGE).A total of 26,33,3 and 15,16,3 alleles were found in 34 Yunnan,24 Tibetan and 6 Xinjiang wheat accessions at Gli-1 and Gli-2 loci,respectively.Some new alleles were identified in Yunnan and Tibetan wheat accessions at Gli-B1,Gli-D1 and Gli-A2 loci.The highest Nei's genetic variation index was detected in B genome as compared to genomes A and D in hexapliod wheat of western China.This indicated that B genome had the highest genetic variation in the three genomes.The Nei's mean genetic variation indexes were 0.6824,0.7471 and 0 for Yunnan,Tibetan and Xinjiang wheats,respectively,which indicated that Yunnan and Tibetan wheats had higher genetic diversity than Xinjiang wheats.