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酚类物质与蝴蝶兰褐变关系初探
引用本文:印芳,葛红,彭克勤,赵伶俐,周玉杰,李秋香.酚类物质与蝴蝶兰褐变关系初探[J].园艺学报,2006,33(5):1137-1140.
作者姓名:印芳  葛红  彭克勤  赵伶俐  周玉杰  李秋香
作者单位:( 中国农业科学院蔬菜花卉研究所, 北京100081; 湖南农业大学植物激素重点实验室, 湖南长沙410128; 西北农林科技大学园艺学院, 陕西杨凌712100 )
基金项目:‘十五’国家‘863’项目(2004AA241200),农业部蔬菜遗传与生理重点开放实验室项目
摘    要: 利用高效液相色谱法对3个褐化程度不同的蝴蝶兰品种A1 (大白花红心) 、B3 (迷你型白花黄心) 和R4 (深红花红心) 进行了9种酚酸定性定量分析, 并研究了以叶片为外植体的初代培养过程中总酚含量的动态变化。结果表明: 绿原酸、邻苯二酚、儿茶酚、咖啡酸及没食子酸、对羟基苯甲酸、香豆酸可能与蝴蝶兰褐变相关, 苯甲酸对蝴蝶兰褐变影响很小; 在褐变过程中, 总酚含量越高, 褐变程度越严重。

关 键 词:蝴蝶兰  褐变  酚酸  总酚
文章编号:0513-353X(2006)05-1137-04
收稿时间:2005-12-08
修稿时间:2005-12-082006-05-17

The Influence of Phenols on Tissue Browning of Phalaenopsis
Yin Fang,Ge Hong,Peng Keqin,Zhao Lingli,Zhou Yujie,Li Qiuxiang.The Influence of Phenols on Tissue Browning of Phalaenopsis[J].Acta Horticulturae Sinica,2006,33(5):1137-1140.
Authors:Yin Fang  Ge Hong  Peng Keqin  Zhao Lingli  Zhou Yujie  Li Qiuxiang
Institution:(Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Key Laboratory of phytohormones of Hunan Agricultural University, Changsha, Hunan 410128, China; College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China)
Abstract:Three varieties of Phalaenopsis spp. including A1, B3 and R4 were used as materials, High Performance Liquid Chromatography (HPLC)was used to analyze phenolic acids of Phalaenopsis spp. qualitatively and quantitatively. Results showed that chlorogenic acid, catechol, catechuic acid, caffeic acid and gallic acid, phydroxybenzoic acid, coumaric acid were correlated with browning, and the influence of benzoic acid on browning was neglectable; The dynamic changes of total phenolic acids contents were studied. Results showed that total phenols were positively correlated with browning.
Keywords:Phalaenopsis  Browning  Phenolic acid  Total phenols
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