Differential antigen recognition by serum antibodies from three bovid hosts of Mycobacterium bovis infection |
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| Affiliation: | 1. Chembio Diagnostic Systems, Inc., 3661 Horseblock Road, Medford, NY 11763, USA;2. Department of Science and Technology-National Research Foundation Centre of Excellence for Biomedical TB Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town 8000, South Africa;3. National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 1920 Dayton Avenue, Ames, IA 50010, USA;1. DST-NRF Centre of Excellence for Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, P.O. Box 241, Cape Town 8000, South Africa;2. Ezemvelo KwaZulu-Natal Wildlife, P.O. Box 25, Mtubatuba, 3935, South Africa;1. Chembio Diagnostic Systems, Inc., 3661 Horseblock Road, Medford, NY 11763, USA;2. SaBio Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, 13005 Ciudad Real, Spain;3. Department of Science and Technology/National Research Foundation Centre of Excellence for Biomedical TB Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town 8000, South Africa;4. National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 1920 Dayton Avenue, Ames, IA 50010, USA;1. Animal and Plant Health Agency—Weybridge, Addlestone, Surrey, United Kingdom;2. AgResearch, Hopkirk Research Institute, Palmerston North, New Zealand;1. DST/NRF Centre of Excellence for Biomedical TB Research/SAMRC Centre for Tuberculosis Research/Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, P.O. Box 241, Cape Town, 8000, South Africa;2. Ezemvelo KZN Wildlife, P.O. Box 25, Mtubatuba, 3935, South Africa |
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| Abstract: | Cattle, bison and buffaloes are susceptible to Mycobacterium bovis, the causative agent for bovine tuberculosis. Accurate and timely identification of infected animals is critical for improved management and control of disease in these species. Bovids develop humoral immune responses to M. bovis infection making serological tests attractive for tuberculosis screening. However, optimization and validation of antibody assays designed for various animal species require understanding of antigen recognition patterns in each target host. The objective of this study was to characterize serological reactivity profiles generated by cattle, American bison, and African buffaloes in tuberculosis. Serum samples from M. bovis-infected animals were tested for the presence of IgM and IgG antibodies to MPB70/MPB83 and CFP10/ESAT6 chimeric proteins using Dual-Path Platform technology. All three host species showed IgG responses of higher magnitude and frequency than IgM responses; further, IgM seroreactivity was limited to MPB70/MPB83, whereas IgG antibodies recognized both test antigens. In cattle, the IgM and IgG responses were elicited mainly by MPB70/MPB83, whereas bison and buffaloes showed similar IgG seroreactivity rates for MPB70/MPB83 and CFP10/ESAT6 antigens. The findings demonstrate distinct patterns of predominant antigen recognition by different bovid species in M. bovis infection. |
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| Keywords: | African buffalo Antibody response Bison Bovine tuberculosis Cattle |
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