A phylogenetic study of Feline Immunodeficiency Virus (FIV) among domestic cats in Turkey |
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| Institution: | 1. Ayd?n Adnan Menderes University, Faculty of Veterinary Medicine, Department of Virology, 09020, Efeler, Aydin, Turkey;2. Ankara University, Faculty of Veterinary Medicine, Department of Virology, 06110, Diskapi, Ankara, Turkey;1. Department of Animal Biology, Biology Institute, State Campinas University, Campinas, São Paulo, Brazil;2. Department of Genetics and Evolution, Microbiology and Immunology, Biology Institute, State Campinas University, Campinas, São Paulo, Brazil;3. Superintendência de Controle de Endemias, Seção Técnica de Pesquisa, Controle deVetores, Campinas, São Paulo, Brazil;4. Department of Clinical Pathology, Faculty of Medical Sciences, University of Campinas, São Paulo, Brazil;1. Department of Veterinary Physiology and Biochemistry, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, 125004, Haryana, India;2. Department of Bio-technology, GLA University, Post-Chaumuhan, Dist., Mathura, Uttar Pradesh, 281 406, India;3. Department of Veterinary Clinical Complex, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, 125004, Haryana, India;1. Department of Virology, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Efeler, Aydin, 09016 Turkey;2. Department of Virology, Faculty of Veterinary Medicine, Ankara University, Diskapi, Ankara, 06110, Turkey;1. Clinical Laboratory and Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland;2. Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland;3. Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland;1. Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium;2. Department of Small Animal Medicine and Clinical Biology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium |
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| Abstract: | Feline Immunodeficiency Virus (FIV) is the most prominent retrovirus in cats. Molecular studies on FIV are of great importance to enable further studies, for example, understanding the pathogenesis and developing improved vaccines. We aimed to elucidate the molecular status of FIV and provide a detailed characterization of FIV in Turkey because at present there is very limited information available in the literature. We also evaluated a potential link between clinical symptoms and FIV subtypes according to results obtained from molecular tests.Whole blood was collected from 200 client-owned domestic cats and molecular diagnosis and characterization was performed. The env, gag and vif gene regions were amplified and sequenced for phylogenetic analysis. We obtained specific amplicons based on bothenvand gag for FIV in 21 cats; only 2 of the 21 positive samples could also be characterized based on the vif gene region. Separate clusters were identified according to previously determined genotyping strategies; however, they were observed in FIV subtype B. The molecular findings of some individual cats were evaluated in conjunction with their clinical symptoms in an attempt to determine potential relationships between the genetic characteristics of FIV and symptoms of disease. As a result, overexpression of the vif gene could be important in leading to serious clinical symptoms.Our results emphasize the necessity of considering FIV in diagnosis and performing the neccesary diagnostics to confirm or rule out FIV infection. The molecular dynamics of FIV should be periodically updated by further analyses to establish a successful prevention strategy. |
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| Keywords: | Feline Immunodeficiency Virus (FIV) Molecular phylogeny Viral infectivity factor (vif) Domestic cats Turkey |
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