High protection of mice against Brucella abortus by oral immunization with recombinant probiotic Lactobacillus casei vector vaccine,expressing the outer membrane protein OMP19 of Brucella species |
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| Affiliation: | 1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, 200241, China;2. College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, China;1. Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;2. Department of Brucella, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Organization, Karaj, Iran;3. Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;1. Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea;2. Division of Applied Life Science (BK21), 2PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea;3. Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea;1. Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Street, Tehran, Iran;2. Department of Molecular Immunology and Vaccine Research, Pasteur Institute of Iran, Pasteur Street, Tehran, Iran;3. Department of Bacteriology, Pasteur Institute of Iran, Pasteur Street, Tehran, Iran;4. Department of Bacterial Vaccine and Antigen Production, Pasteur Institute of Iran, Karaj, Iran |
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| Abstract: | Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country. Vaccination is the most effective way to control brucellosis. The recombinant vector vaccines are promising candidates for immunization in humans and animals. In this study, the gene encoding OMP19 antigen was primarily amplified and cloned into an expression vector called pT1NX, and then transformed to L. casei cell via electroporation technique. The expression was confirmed using specific antibody against the recombinant protein via immunological screening tests such as western blot and immunofluorescence assay. Finally, recombinant L. casei was orally fed to mice and the results were further recorded, indicating that the mice group which received OMP19 through L. casei based vaccine represented a very good general and mucosal immune responses protective against challenges with virulent B. abortus 544 strain compared with negative control recipient groups. Therefore, the vaccine produced in this research plan can be a very good candidate for protection against brucellosis. |
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| Keywords: | Brucellosis Vector vaccine OMP19 |
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