Genotyping and antimicrobial susceptibility of Clostridium perfringens isolated from dromedary camels,pastures and herders |
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| Affiliation: | 1. Al Ahsa Veterinary Diagnostic Laboratory, Ministry of Environment, Water and Agriculture, Saudi Arabia;2. Veterinary Serum and Vaccine Research Institute, Ministry of Agriculture, Cairo, Egypt;3. Department of Animal Medicine, Division of Infectious Diseases, Faculty of Veterinary Medicine, Zagazig University, Zagazig City, Sharkia Province, Egypt;4. Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island, Canada;5. General Organization of Veterinary Services, Ministry of Agriculture, Cairo, Egypt;6. Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture, Saudi Arabia;7. Hygiene and Preventive Medicine Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh, Egypt;1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, 200241, China;2. College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, China;1. College of Life Science and Engineering, Foshan University, Foshan 528231, Guangdong, China;2. Key Laboratory for Preventive Research of Emerging Animal Diseases, Foshan University, Foshan 528231, Guangdong, China;3. Shenzhen Carla Pet Hospital Co., LTD, China;4. Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou 310058, Zhejiang, China;1. École Nationale Supérieure Vétérinaire d''Alger, Algérie, Laboratoire de Recherche Gestion des Ressources Animales Locales (GRAL), Algeria;2. UMR BIPAR, Ecole Nationale Vétérinaire d''Alfort, Anses, INRA, Université Paris-Est, Maisons-Alfort, France;3. Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA;1. Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand;2. Microbiology Laboratory, Veterinary Diagnostic Center, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand;3. Faculty of Public Health, Thammasat University, Pathumthani, 12121, Thailand;4. Zoetis (Thailand) Limited, 323 United Building, 46th Floor, Silom Road, Bangkok, 10400, Thailand;5. Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand;1. Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243122, India;2. Veterinary Public Health Laboratory, Indian Veterinary Research Institute, 37, Belgachia Road, Kolkata, 700037, India;3. Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, Kerala, 680651, India;4. Division of Bacteriology, National Institute of Cholera and Enteric Diseases, Beliaghata, Kolkata, 700010, India;5. Division of Livestock Products Technology, Indian Veterinary Research Institute, Izatnagar, 243122, India;6. Division of Pathology, Indian Veterinary Research Institute, Izatnagar, 243122, India;1. Key Laboratory of Prevention and Control Agents for Animal Bacteriosis, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan, China;2. Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan, China |
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| Abstract: | The present study aimed to isolate and genotype C. perfringens from healthy and diarrheic dromedary camels, pastures and herders; and to evaluate and compare antimicrobial susceptibility of the isolates. A total of 262 (56.3%) C. perfringens isolates were recovered from 465 samples of healthy and diarrheic dromedary camels, pastures and herders. C. perfringens type A (75.2%), type B (4.2%), type C (13.7%) and type D (6.9%) were detected. C. perfringens type A with only cpa+ gene was found in 191 (72.9%) isolates and with cpa+ associated cpb2+ was found only in 6 (2.3%) isolates. None of the isolates were positive for cpe and iap genes.The highest antimicrobial resistance (82.8%) was observed to ceftiofur with MIC50 and MIC90 values of <64 and ≥256 μg/mL, respectively, followed by penicillin G (72.9%) and erythromycin (61.5%). The lowest resistance (1.9%) was observed for doxycycline with MIC50 and MIC90 values of <1 and 4 μg/mL, respectively, followed by florfenicol (5.3%) and clindamycin (12.2%). In conclusion, C. perfringens type A with cpa+ gene was the most prevalent toxin type isolated in this study. The majority of the isolates were resistant to at least one of the ten antimicrobials tested. Antimicrobial resistance patterns of C. perfringens isolates provide further evidence on the emergence of multiple-drug resistant C. perfringens. Therefore, the dissemination of surveillance programs to monitor and control C. perfringens in dromedary camels is required. |
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| Keywords: | Dromedary camel Genotyping Antimicrobial resistance |
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