Sequence characterized amplified region (SCAR) analysis on DNA from the three medicinal Echinacea species |
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| Authors: | Adinolfi Barbara Chicca Andrea Martinotti Enrica Breschi Maria C Nieri Paola |
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| Affiliation: | Department of Psychiatry, Neurobiology, Pharmacology and Biotechnology, University of Pisa, Italy. badinolfi@yahoo.it |
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| Abstract: | In our previous study, RAPD (Random Amplified Polymorphic DNA) analysis revealed species-specific markers for three medicinal Echinacea species (Asteraceae): E. angustifolia DC., E. pallida (Nutt.) Nutt. and E. purpurea (L.) Moench. In the present work, we have converted a RAPD marker (750 bp) for E. purpurea into a SCAR (Sequence Characterized Amplified Region) marker. SCAR-PCR, in fact, revealed the expected amplicon (330 bp) only in E. purpurea and not in the other two species, giving further evidence for differences in medicinal Echinacea spp. genome and confirming a greater similarity between E. pallida and angustifolia. |
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| Keywords: | Echinacea species SCAR (Sequence Characterized Amplified Region) Genetic differentiation |
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