用AFLP标记一个来自偏凸山羊草的抗条锈病新基因 YrG775

 对小麦抗病种质贵农775与西农97148的F2后代人工接种CY32条锈病菌进行抗性鉴定，通过卡方检测抗感病单株分离比例确定贵农775携带两对重叠抗条锈病基因。从128个AFLP引物组合中筛选到与其中一个抗病基因YrG775共分离的多态性标记M8P151200bp，该标记仅能在原始亲本偏凸山羊草中检测到。由于已知来源于偏凸山羊草的Yr17苗期不抗CY32条锈病菌，所以根据抗性鉴定和分子生物学试验结果，推断YrG775很可能是一个来自偏凸山羊草并与已知抗条锈病基因都不同的新基因。

Tagging a Novel Yellow Rust Resistance Gene YrG775 Derived from Aegilops ventricosa with AFLP Marker

In this study, the F2 populations crossing by Xinong97148×Guinong775 was Inoculated with the pathology strains CY32 to test their resistance. The Chi-square tests results showed the yellow rust resistance of guinong775 was controlled by two duplicate genes. Screened 128 AFLP primer combinations, the primer combination M8P15 had a polymorphic band about 1200bp linked to the YrG775 (temporarily designed), one of the two duplicate gene. F2 segregations analysis confirmed the band co-segregated with this gene and only Aegilops ventricosa one of the original parents of guinong 775 had this polymorphic band. Since the Yr17 the only yellow rust resistance gene known to be derived from Aegilops ventricosa had no resistance to the pathology strains CY32, the gene YrG775 identified in this paper might to be a novel yellow rust resistance gene.