百合鳞片离体培养的研究

以'布鲁拉诺'、'提伯'和'索蚌'3个百合品种的鳞片为外植体,比较了不同的消毒方法、不同品种、不同取材部位及接种方法对百合鳞片离体培养的影响.结果表明,最佳的消毒方法是0.1%升汞消毒8 min,污染率较低,成活率最高,可达90.9%,且采用小鳞茎整体消毒可以有效降低污染率.不同百合品种分化小鳞茎的能力有较大差异,分化能力依次为'布鲁拉诺'＞'索蚌'＞'提伯'.百合鳞片不同部位分化小鳞茎的能力从大到小依次为下部、中部、上部.近轴面向上接种的鳞片分化能力强于远轴面向上,小鳞茎再生率最高,可达100%.

Study on Bulb Scale Culture in Vitro of Lily

The bulb scales of three lily cultivars 'Brunello', 'Tiber' and 'Sorbonne' were used as explants in the experiment.The effect of different methods of sterilization,different cultivars,different parts and orientation of scales inoculated on culturing in vitro of bulb scales in lily were compared.The results showed that the best method of sterilization was 0.1% HgCl2 with 8 minutes.The rate of contamination was lower and the rate of survival was up to 90.9%.The rate of contamination could be reduced using the sterilization method for the whole bulb.The differentiation capability of bulblets among lily cultivars was different obviously.The capability from strong to weak was 'Brunello','Sorbonne' and 'Tiber'.The capablity of different parts of bulb scale to differentiate bulblets from strong to weak was underside, middle and upside.The bulblet regeneration was more when the bulb scales were inoculated upwards with the rate up to 100%.